Abstract 4337: Selection of DNA aptamers for an ovarian cancer cell line using high-throughput sequencing.

适体 指数富集配体系统进化 卵巢癌 流式细胞术 计算生物学 癌症研究 分子生物学 DNA测序 基因敲除 SELEX适体技术 生物 化学 DNA 癌症 细胞培养 遗传学 基因 核糖核酸
作者
Rebecca J. Whelan,Arvinder Kapur,Mildred Felder,Jeff Nie,Manish S. Patankar
出处
期刊:Cancer Research [American Association for Cancer Research]
卷期号:73 (8_Supplement): 4337-4337 被引量:1
标识
DOI:10.1158/1538-7445.am2013-4337
摘要

Abstract The mucin, MUC16, facilitates metastasis and immuneprotection of ovarian tumors. Therefore, agents that effectively target MUC16 expressing ovarian cancer cells will be useful for diagnostic and therapeutic applications. Here, we have utilized the Cell-based Systematic Evolution of Ligands by Exponential Enrichment (cell-SELEX) approach to generate high affinity single-stranded DNA (ssDNA) aptamers as agents for detection and treatment of MUC16-positive ovarian tumors. An ssDNA aptamer library composed of ∼100 trillion sequences was subjected to ten iterative rounds of negative and positive selection using the MUC16-knockdown and MUC16-positive ovarian cancer cell line, OVCAR-3. Aptamers from each round were amplified by asymmetric PCR and subjected to high throughput NextGen sequencing. Eight ssDNA aptamers enriched through the selection process were identified by bioinformatics analysis and their selectivity and affinity for MUC16-positive cells was determined by flow cytometry. Two of these aptamers (Apt-1 and Apt-8) showed significant binding to the MUC16-positive OVCAR-3 with a Kd of 24 and 28 nM, respectively. In contrast, Apt-1 and Apt-8 only weakly recognized the MUC16-knockdown OVCAR-3 cells. M-Fold analysis indicated that Apt-1 and Apt-8 had defined secondary structures resulting from ordered base pairing of the ssDNA. The inclusion of NextGen sequencing techniques has therefore allowed rapid identification of theranostic aptamers from an exhaustive library of ssDNA sequences. This study provides a streamlined method for development of theranostic aptamers that can be used to recognize and target MUC16 expressing ovarian cancer cells. Citation Format: Rebecca J. Whelan, Arvinder Kapur, Mildred Felder, Jeff Nie, Manish S. Patankar. Selection of DNA aptamers for an ovarian cancer cell line using high-throughput sequencing. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4337. doi:10.1158/1538-7445.AM2013-4337

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
David完成签到 ,获得积分10
刚刚
小小一个应助和谐的梦蕊采纳,获得10
1秒前
1秒前
hhhuan完成签到,获得积分10
2秒前
3秒前
孟浩关注了科研通微信公众号
3秒前
晓天完成签到,获得积分10
4秒前
泽烺木完成签到,获得积分10
6秒前
落寞白曼完成签到,获得积分10
6秒前
人间不清醒完成签到,获得积分20
6秒前
欢欢发布了新的文献求助10
6秒前
快乐的奕涵完成签到,获得积分10
7秒前
zongzi12138完成签到,获得积分0
8秒前
8秒前
王哈哈完成签到,获得积分10
8秒前
香蕉觅云应助人间不清醒采纳,获得30
10秒前
13秒前
14秒前
bzdjsmw完成签到 ,获得积分10
14秒前
WebCasa应助旦皋采纳,获得10
14秒前
路易斯完成签到,获得积分10
15秒前
颜愫发布了新的文献求助10
15秒前
萌萌完成签到,获得积分10
16秒前
研友_X89o6n完成签到,获得积分10
18秒前
Ther发布了新的文献求助10
20秒前
哈哈哈完成签到,获得积分10
21秒前
23秒前
诚心的初露完成签到,获得积分10
23秒前
lyb完成签到 ,获得积分10
25秒前
风中方盒完成签到,获得积分20
25秒前
布丁圆团完成签到,获得积分10
26秒前
yikeshu完成签到,获得积分10
26秒前
Zoe完成签到 ,获得积分10
27秒前
29秒前
星辰大海应助do0采纳,获得10
30秒前
tt完成签到 ,获得积分10
31秒前
浅辰完成签到,获得积分10
32秒前
大气萤完成签到,获得积分20
33秒前
33秒前
我ppp完成签到 ,获得积分10
33秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029