Endothelial Progenitor Cells Modulate the Phenotype of Smooth Muscle Cells and Increase Their Neointimal Accumulation Following Vascular Injury

新生内膜 祖细胞 细胞生物学 细胞生长 再狭窄 医学 内皮祖细胞 干细胞 新生内膜增生 生物 癌症研究 化学 内科学 支架 生物化学
作者
Sebastian F. Mause,Elisabeth Ritzel,Annika Deck,Felix Vogt,Elisa A. Liehn
出处
期刊:Thrombosis and Haemostasis [Georg Thieme Verlag KG]
卷期号:122 (03): 456-469 被引量:13
标识
DOI:10.1055/s-0041-1731663
摘要

Smooth muscle cells (SMCs) are the main driver of neointima formation and restenosis following vascular injury. In animal models, endothelial progenitor cells (EPCs) accelerate endothelial regeneration and reduce neointima formation after arterial injury; however, EPC-capture stents do not reduce target vessel failure compared with conventional stents. Here we examined the influence of EPCs on features of SMCs pivotal for their impact on injury-induced neointima formation including proliferation, migration, and phenotype switch. EPCs, their conditioned medium, and EPC-derived microparticles induced proliferation of SMCs while limiting their apoptosis. In transwell membrane experiments and scratch assays, EPCs stimulated migration of SMCs and accelerated their recovery from scratch-induced injury. Treatment of SMCs with an EPC-derived conditioned medium or microparticles triggered transformation of SMCs toward a synthetic phenotype. However, co-cultivation of EPCs and SMCs enabling direct cell-cell contacts preserved their original phenotype and protected from the transformative effect of SMC cholesterol loading. Adhesion of EPCs to SMCs was stimulated by SMC injury and reduced by blocking CXCR2 and CCR5. Interaction of EPCs with SMCs modulated their secretory products and synergistically increased the release of selected chemokines. Following carotid wire injury in athymic mice, injection of EPCs resulted not only in reduced neointima formation but also in altered cellular composition of the neointima with augmented accumulation of SMCs. EPCs stimulate proliferation and migration of SMCs and increase their neointimal accumulation following vascular injury. Furthermore, EPCs context-dependently modify the SMC phenotype with protection from the transformative effect of cholesterol when a direct cell-cell contact is established.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
十八发布了新的文献求助10
刚刚
刚刚
刚刚
kf033完成签到,获得积分10
2秒前
4秒前
宓不评完成签到 ,获得积分20
5秒前
wanci应助巴基斯坦农民采纳,获得10
7秒前
7秒前
陈秋发布了新的文献求助10
9秒前
乐在奇中完成签到,获得积分10
10秒前
12秒前
12秒前
忍无可任女士完成签到 ,获得积分10
12秒前
air完成签到 ,获得积分10
13秒前
二木发布了新的文献求助10
13秒前
zhangpeiqi发布了新的文献求助10
14秒前
liusoojoo完成签到,获得积分10
17秒前
娃哈哈完成签到,获得积分10
19秒前
lgh19950929发布了新的文献求助10
19秒前
wanci应助初初遇你采纳,获得10
20秒前
22秒前
22秒前
小蘑菇应助cannon8采纳,获得10
23秒前
yiheng完成签到,获得积分10
23秒前
61414发布了新的文献求助10
23秒前
bkagyin应助坦率的丹琴采纳,获得10
23秒前
25秒前
25秒前
夏天完成签到,获得积分10
26秒前
小猪坨完成签到,获得积分10
27秒前
28秒前
夏天发布了新的文献求助10
29秒前
安紊完成签到,获得积分10
29秒前
娃哈哈发布了新的文献求助10
30秒前
KatzeBaliey完成签到,获得积分10
30秒前
光亮的太阳完成签到,获得积分10
31秒前
32秒前
CipherSage应助高大凌寒采纳,获得200
34秒前
Lori完成签到,获得积分10
34秒前
星辰大海应助重要的天空采纳,获得10
35秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Threaded Harmony: A Sustainable Approach to Fashion 810
Pharmacogenomics: Applications to Patient Care, Third Edition 800
A Dissection Guide & Atlas to the Rabbit 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3082501
求助须知:如何正确求助?哪些是违规求助? 2735655
关于积分的说明 7538441
捐赠科研通 2385263
什么是DOI,文献DOI怎么找? 1264761
科研通“疑难数据库(出版商)”最低求助积分说明 612786
版权声明 597665