生物
寄生虫血症
流式细胞术
染色
恶性疟原虫
吉姆萨染色
核酸
荧光染料
寄生虫寄主
疟原虫(生命周期)
细胞仪
分子生物学
疟疾
生物化学
实时聚合酶链反应
免疫学
遗传学
万维网
基因
计算机科学
作者
Shinji Izumiyama,Mako Omura,Tomohiko Takasaki,Hiroshi Ohmae,Hiroko Asahi
标识
DOI:10.1016/j.exppara.2008.10.008
摘要
Reliable analytical techniques to test growth-promoting and antimalarial efficacy on plasmodia are very important. Flow cytometry (FCM) offers the possibility to study developmental stages of intraerythrocytic growth of malaria parasites using nucleic acid staining. To analyze the growth of Plasmodium falciparum SYBR Green I was introduced as an intercalating dye with FCM for the 488nm line of an argon laser. Procedures employing FCM, including fixatives, dye concentrations, dilution buffer, and staining period, were optimized to simplify the method. FCM as described here allows parasitemia and parasites of different stages to be quantified according to the DNA content. The proportion of parasitized erythrocytes estimated by FCM and the Giemsa method agreed with determination by parasite lactate dehydrogenase. The protocol was extended to merozoite counting as a sensitive assay of growth inhibition of the parasite.
科研通智能强力驱动
Strongly Powered by AbleSci AI