胞吐
内体
细胞生物学
生物
高尔基体
小泡
脂质双层融合
钙
细胞器
分泌泡
胞浆
囊泡融合
内吞作用
膜
生物物理学
内质网
细胞
生物化学
化学
突触小泡
细胞内
有机化学
酶
作者
Jyoti K. Jaiswal,Norma W. Andrews,Sanford M. Simon
标识
DOI:10.1083/jcb.200208154
摘要
Similar to its role in secretory cells, calcium triggers exocytosis in nonsecretory cells. This calcium-dependent exocytosis is essential for repair of membrane ruptures. Using total internal reflection fluorescence microscopy, we observed that many organelles implicated in this process, including ER, post-Golgi vesicles, late endosomes, early endosomes, and lysosomes, were within 100 nm of the plasma membrane (in the evanescent field). However, an increase in cytosolic calcium led to exocytosis of only the lysosomes. The lysosomes that fused were predominantly predocked at the plasma membrane, indicating that calcium is primarily responsible for fusion and not recruitment of lysosomes to the cell surface.
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