萝卜
黄瓜
酸性磷酸酶
化学
酶
磷酸酶
生物化学
磷酸盐
水解
酶分析
阿皮拉酶
植物
生物
作者
Luciane Almeri Tabaldi,Raquel Ruppenthal,Luciane Belmonte Pereira,Denise Cargnelutti,Jamile F. Gonçalves,Vera Maria Morsch,Maria Rosa Chitolina Schetinger
出处
期刊:Ciencia Rural
[SciELO]
日期:2008-04-10
卷期号:38 (3): 650-657
被引量:7
标识
DOI:10.1590/s0103-84782008000300009
摘要
Acid phosphatases (3.1.3.2) are a group of enzymes widely distributed in nature, which catalyze the hydrolysis of a variety of phosphate esters in the pH range of 4-6. We confirmed the presence of acid phosphatases in seedlings of cucumber (Cucumis sativus), radish (Raphanus sativus) and rocket salad (Eruca vesicaria) under different assay conditions using a rapid and simple preparation. The results showed that the optimum pH and temperature used for all species were close to 5.5 and 35°C, respectively. The enzyme was inhibited by molybdate, fluoride, azide, levamisole, orthovanadate, Zn2+ and Cu2+. Suramin had no effect on enzyme activity. The acid phosphatase from cucumber, radish and rocket salad hydrolyzed a wide variety of phosphate esters and the highest activity was observed with PPi, ATP and GTP. These results demonstrate that the enzyme investigated in this study is different from well known ester phosphate cleaving plant enzymes (apyrase and inorganic pyrophosphatases) and this preparation could be a useful tool to future toxicological studies and to study initially all isoforms of acid phosphatase.
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