脑膜炎奈瑟菌
色谱法
安培法
奈瑟菌科
化学
水解
离子色谱法
奈瑟菌
酸水解
血清型
离子交换
定量分析(化学)
多糖
细菌
微生物学
生物化学
生物
离子
抗生素
电极
遗传学
物理化学
电化学
有机化学
作者
Stefano Ricci,Angela Bardotti,Sandro D’Ascenzi,Neil Ravenscroft
出处
期刊:Vaccine
[Elsevier]
日期:2001-02-01
卷期号:19 (15-16): 1989-1997
被引量:48
标识
DOI:10.1016/s0264-410x(00)00427-8
摘要
A new method for the quantitative determination of Neisseria meningitidis group A (MenA) capsular polysaccharide (CPS) has been developed. The method is based on trifluoracetic acid (TFA) hydrolysis of the CPS (2 M at 80 degrees C for 3 h), followed by chromatographic separation and quantification of the liberated mannosamine-6-phosphate from the area of the peak obtained using an IonPac AS11 column coupled to the sensitive pulsed amperometric detector ED40. The highly selective nature of this method circumvents the interference problems associated with the classical method based on a colorimetric assay for phosphorus. Provided that suitable hydrolysis conditions can be found, this chromatographic approach might be applicable to the quantification of other bacterial antigens containing phosphorylated sugars such as meningococcal groups H, L, X and Z, and pneumococcal serotypes 6, 10A and 19.
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