亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Improved blood–brain barrier distribution: Effect of borneol on the brain pharmacokinetics of kaempferol in rats by in vivo microdialysis sampling

微透析 药代动力学 体内 药理学 冰片 血脑屏障 医学 血液取样 颈静脉 化学 内科学 病理 中枢神经系统 中医药 生物 替代医学 生物技术
作者
Qi Zhang,Dong Wu,Juan Wu,Yvonne Ou,Chunlei Mu,Bo Han,Qunlin Zhang
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:162: 270-277 被引量:74
标识
DOI:10.1016/j.jep.2015.01.003
摘要

Kaempferol (KA) exists in a variety of herbal medicines. In vitro and in vivo studies have focused on the anti-Alzheimer effect of KA. However, little is known about its brain pharmacokinetic profile. The accumulated amount of KA in brain is very low because of the protection of blood–brain barrier (BBB). Borneol (BO) is a classical aromatic refreshing traditional Chinese medicine and commonly used as an adjuvant component of traditional Chinese medicines (e.g. compound Danshen dropping pills) in the treatment of cardiovascular and cerebrovascular diseases. According to the basic theories of traditional Chinese medicine, BO is called an “upper guiding drug”, which can guide other components to the targeting tissues or organs in the upper part of the body, especially in the brain. The probes for blood and brain sampling were implanted within the jugular vein/right atrium and right hippocampus of SD rats, respectively. Rats were intravenous administered of KA (25 mg/kg) alone or combined with BO (15, 30 mg/kg) via caudal vein. The blood and brain microdialysates were collected every 15 min for 180 min and every 30 min for 180−300 min. A selective and sensitive high performance liquid chromatography–chemiluminescence method was developed for the determination of unbound KA in rat blood and brain microdialysates, which can be converted to their actual free-form concentrations based on the in vivo relative recoveries of KA across microdialysis probes. KA quickly crossed the BBB to enter the extracellular fluid of hippocampus and reached the maximum concentration of 0.11 μg/mL within 30 min. The brain bioavailability and brain delivery of KA evidently increased with the co-administration of 15 and 30 mg/kg of BO. The AUC0–inf of KA in brain increased 1.84 and 2.19 times, and the Cmax of KA in brain increased 2.09 and 3.18 times than that without BO, respectively. In addition, the brain-to-blood distribution ratio of KA increased by 48.68% and 57.97% compared with that without BO. However, no significant difference in the T1/2 of unbound KA in blood aserved between three groups. BO can enhance the BBB permeability and improve the transportation of KA to brain. The dose-dependent effect of BO on the brain pharmacokinetic parameters of KA was observed. This co-administration strategy can be designed to enhance the brain accumulation of other neuropsychiatric medications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Andy_2024完成签到,获得积分10
2秒前
成就的孤晴完成签到 ,获得积分10
8秒前
垚祎完成签到 ,获得积分10
11秒前
16秒前
传奇3应助科研通管家采纳,获得10
23秒前
32秒前
45秒前
yaoyao发布了新的文献求助10
50秒前
1分钟前
Jack80发布了新的文献求助50
1分钟前
科研王者发布了新的文献求助10
1分钟前
1分钟前
1分钟前
zxr123关注了科研通微信公众号
2分钟前
2分钟前
2分钟前
Chenzr发布了新的文献求助10
2分钟前
Lsh173373完成签到,获得积分10
2分钟前
搜集达人应助Jack80采纳,获得50
2分钟前
爆米花应助科研通管家采纳,获得10
2分钟前
2分钟前
2分钟前
zxr123发布了新的文献求助10
2分钟前
2分钟前
舒心的晟睿完成签到,获得积分10
2分钟前
2分钟前
Jack80发布了新的文献求助50
2分钟前
豆包完成签到,获得积分10
3分钟前
3分钟前
3分钟前
3分钟前
3分钟前
阿智发布了新的文献求助10
3分钟前
3分钟前
3分钟前
3分钟前
隐形曼青应助西瓜撞地球采纳,获得10
3分钟前
3分钟前
3分钟前
dilli完成签到 ,获得积分10
4分钟前
高分求助中
歯科矯正学 第7版(或第5版) 1004
Semiconductor Process Reliability in Practice 1000
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 600
GROUP-THEORY AND POLARIZATION ALGEBRA 500
Mesopotamian divination texts : conversing with the gods : sources from the first millennium BCE 500
Days of Transition. The Parsi Death Rituals(2011) 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3234555
求助须知:如何正确求助?哪些是违规求助? 2880908
关于积分的说明 8217319
捐赠科研通 2548507
什么是DOI,文献DOI怎么找? 1377792
科研通“疑难数据库(出版商)”最低求助积分说明 647999
邀请新用户注册赠送积分活动 623347