类毒素
检出限
荧光
破伤风
化学
分析化学(期刊)
色谱法
医学
病毒学
光学
物理
接种疫苗
作者
Piaopiao Chen,Yunjin Bai,Shiyuan Tang,Nian Wang,Yaqin He,Ke Huang,Jin Huang,Binwu Ying,Yu Cao
出处
期刊:Nano Letters
[American Chemical Society]
日期:2022-02-04
卷期号:22 (4): 1710-1717
被引量:43
标识
DOI:10.1021/acs.nanolett.1c04818
摘要
Here, we report a simple aptamer-based toxoid test with both fluorescence and binary visual readouts. This test is established based on our recent finding that CdTe quantum dots could differentiate DNA templated Cu NPs from Cu2+. Through the further integration with enzyme-free triple parallel hybridization chain reaction, cation exchange reaction, and inkjet printing, we demonstrated specific detection of tetanus toxoid with a limit-of-detection (LOD) of 0.25 fg/mL using fluorescence readout. Using color- and distance-based binary visual readouts, we were able to achieve LODs of 10 fg/mL and 1 fg/mL, respectively. The quantitative test results for tetanus toxoid using both fluorescence and visual readouts were successfully validated in 84 clinical serum samples. Moreover, our strategy also enabled accurate monitoring of tetanus toxoid levels in patients before and after drug treatment. On the basis of our clinical test results, we recommend a cutoff value of 5 fg/mL for tetanus infection.
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