脱氧核酶
分子信标
小RNA
荧光
化学
生物物理学
小发夹RNA
基质(水族馆)
检出限
纳米技术
计算生物学
寡核苷酸
组合化学
DNA
核糖核酸
生物化学
生物
基因
材料科学
物理
色谱法
生态学
量子力学
作者
Chenxin Fang,Yuxing Yang,Shuhao Zou,Ping Ouyang,Qing Yang,Jialun Han,Haiyu Li,Zhencui Wang,Jie Du
出处
期刊:Biosensors
日期:2022-04-27
卷期号:12 (5): 276-276
被引量:4
摘要
Although miRNAs exist in small quantities in the human body, they are closely related to the abnormal expression of genes in diseases such as tumors. Therefore, sensitive detection of miRNAs is very important for the prevention and treatment of various tumors and major diseases. The purpose of this study is to develop a label-free sensing strategy based on the co-action of double-hairpin molecular beacons and deoxyribozymes (DNAzymes) for highly sensitive detection of miRNA-21. The target miRNA-21 promotes the assembly of DNAzyme with a complete catalytic core region. At the presence of Mg2+, DNAzyme cuts a substrate into short chains, which open the double hairpin molecular beacon, and then form G-quadruplexs at both ends, specifically binding more ThT to generate a amplified fluorescent signal. The cut substrate will be replaced by the uncut ones in the next stage, increasing the concentration of reactants, and thus further improving the fluorescence intensity. This DNAzyme assisted double hairpin molecular beacon has a certain degree of discrimination for substances with single base mismatches, and the detection limit of miRNA-21 is 0.13 pM, lower than that of the many other analysis. Further, this detection has good selectivity and sensitivity in serum. Therefore, this strategy provides a simple, fast and low-cost platform for the sensitive detection of miRNA-21, having potential applications in early cancer diagnosis.
科研通智能强力驱动
Strongly Powered by AbleSci AI