Effects of different biological enzymes on preparation of tea seed oil diglyceride

Excessive consumption of traditional oil, mainly triglyceride, is the main factor leading to obesity, whereas diglyceride in tea seed oil has anti-obesity properties. Lipozyme RM IM, Lipozyme TL IM and Novozym 435 were used to study the catalytic transformation of tea seed oil into diglycerides by hydrolysis method. The yield of diglycerides prepared by Novozym 435 was 15.37%, which was significantly higher than the other two enzymes. The effects of time on the catalytic conversion of tea seed oil diglycerides by three biological enzymes were investigated. The values for acid, iodine and peroxide from tea seed oil diglycerides were measured at different reaction times. It was found that the acid value and peroxide value of Novozym 435 hydrolysate were higher than the other two biological enzymes, while the iodine value of the three biological enzymes changed little with time. Finally, Novozym 435 was selected as the biological enzyme for the preparation of tea seed oil diglycerides, and the catalytic transformation of tea seed oil diglycerides of Novozym 435 was characterized by infrared spectroscopy, and the target product was determined and the content was high.