Physiological and biochemical responses to caffeine and microplastics in Mytilus galloprovincialis

贻贝 微塑料 氧化应激 活力测定 过氧化氢酶 金属硫蛋白 超氧化物歧化酶 谷胱甘肽 脂质过氧化 化学 生物化学 唾液 生物 环境化学 细胞 生态学 基因
作者
Federica Impellitteri,Kateryna Yunko,Viktoria Martyniuk,Tetiana Matskiv,Sofia A. Lechachenko,Vira Khoma,Alla Mudra,Giuseppe Piccione,Оksana Stoliar,Caterina Faggio
出处
期刊:Science of The Total Environment [Elsevier]
卷期号:890: 164075-164075 被引量:34
标识
DOI:10.1016/j.scitotenv.2023.164075
摘要

Caffeine (Caff) is one of the most widely used substances in the human diet and a well-recognized drug. Its input into surface waters is remarkable, but biological effects on aquatic organisms are unclear, particularly in combination with pollutants of suspected modulatory activity, like microplastics (MP). The aim of this study was to reveal the impact on the marine mussel Mytilus galloprovincialis (Lamark, 1819) of Caff (20.0 μg L-1) in the environmentally relevant combination (Mix) with MP 1 mg L-1 (size 35-50 μm) after the exposure for 14 days. Untreated and exposed to Caff and MP separately groups were also examined. Cell viability and cell volume regulation in hemocytes and digestive cells, as well as the indexes of oxidative stress, glutathione (GSH/GSSG) and metallothioneins levels, and caspase-3 activity in digestive gland were assessed. MP and Mix reduced Mn-superoxide dismutase, catalase, and glutathione S-transferase activities and level of lipid peroxidation, but increased the digestive gland cell viability, GSH/GSSG ratio (by 1.4-1.5-fold), metallothioneins level and their Zn content, while Caff did not affect oxidative stress indexes and metallothionein-related Zn chelation. Protein carbonyls were not targeted in all exposures. The distinguishing feature of the Caff group was the decline (2-fold) in caspase-3 activity and low cell viability. The multi-stress effect of Mix was shown by the worsening of the volume regulation of digestive cells and confirmed by discriminant analysis of biochemical indexes. The special capabilities of M. galloprovincialis as a sentinel organism make it an excellent bio-indicator reflecting the multi-stress effects in sub-chronic exposures to potentially harmful substances. The identification of the modulation of individual effects in combined exposure increases the need to base monitoring programs on studies of multi-stress effects in sub-chronic exposures.
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