Harnessing Mechanical Stress with Viscoelastic Biomaterials for Periodontal Ligament Regeneration

自愈水凝胶 机械转化 牙周纤维 粘弹性 应力松弛 细胞外基质 生物物理学 材料科学 机械生物学 生物医学工程 再生(生物学) 焦点粘着 化学 细胞生物学 细胞 复合材料 蠕动 牙科 高分子化学 生物化学 医学 生物
作者
Jiu‐Jiu Zhang,Xuan Li,Yi Tian,Jie‐Kang Zou,Dian Gan,Dao‐Kun Deng,Chen Jiao,Yuan Yin,Bei‐Min Tian,Rui‐Xin Wu,Fa‐Ming Chen,Fa‐Ming Chen
出处
期刊:Advanced Science [Wiley]
卷期号:11 (18) 被引量:18
标识
DOI:10.1002/advs.202309562
摘要

Abstract The viscoelasticity of mechanically sensitive tissues such as periodontal ligaments (PDLs) is key in maintaining mechanical homeostasis. Unfortunately, PDLs easily lose viscoelasticity (e.g., stress relaxation) during periodontitis or dental trauma, which disrupt cell–extracellular matrix (ECM) interactions and accelerates tissue damage. Here, Pluronic F127 diacrylate (F127DA) hydrogels with PDL‐matched stress relaxation rates and high elastic moduli are developed. The hydrogel viscoelasticity is modulated without chemical cross‐linking by controlling precursor concentrations. Under cytomechanical loading, F127DA hydrogels with fast relaxation rates significantly improved the fibrogenic differentiation potential of PDL stem cells (PDLSCs), while cells cultured on F127DA hydrogels with various stress relaxation rates exhibited similar fibrogenic differentiation potentials with limited cell spreading and traction forces under static conditions. Mechanically, faster‐relaxing F127DA hydrogels leveraged cytomechanical loading to activate PDLSC mechanotransduction by upregulating integrin–focal adhesion kinase pathway and thus cytoskeletal rearrangement, reinforcing cell–ECM interactions. In vivo experiments confirm that faster‐relaxing F127DA hydrogels significantly promoted PDL repair and reduced abnormal healing (e.g., root resorption and ankyloses) in delayed replantation of avulsed teeth. This study firstly investigated how matrix nonlinear viscoelasticity influences the fibrogenesis of PDLSCs under mechanical stimuli, and it reveals the underlying mechanobiology, which suggests novel strategies for PDL regeneration.
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