微泡
肺结核
结核分枝杆菌
蛋白质组学
发病机制
定量蛋白质组学
载脂蛋白E
外体
生物
病菌
医学
小RNA
免疫学
基因
疾病
生物化学
内科学
病理
作者
Mingrui Wu,Wei Wang,Caiting Yang,Han Jie,Hai Liu,Lingran Qiao,Huiping Duan,Li Xing,Qin-dong Liu,Dong Li,Quanhong Wang,Lin Zuo
出处
期刊:Tuberculosis
[Elsevier]
日期:2023-07-01
卷期号:141: 102359-102359
被引量:6
标识
DOI:10.1016/j.tube.2023.102359
摘要
Increasing prevalence of drug-resistant tuberculosis (DR-TB) poses a major challenge to the early detection and effective control of tuberculosis (TB). Exosomes carrying proteins and nucleic acid mediate intercellular communication between host and pathogen including Mycobacterium tuberculosis. However, molecular events of exosomes indicating the status and development of DR-TB remain unknown. This study determined the proteomics of exosome in DR-TB and explored the potential pathogenesis of DR-TB.Plasma samples were collected from 17 DR-TB patients and 33 non-drug-resistant tuberculosis (NDR-TB) patients using grouped case-control study design. After exosomes of plasma were isolated and confirmed by compositional and morphological measurement for exosomal characteristics, a label-free quantitative proteomics of exosomes was performed and differential protein components were determined via bioinformatics analysis.Compared with the NDR-TB group, we identified 16 up-regulated proteins and 10 down-regulated proteins in the DR-TB group. The down-regulated proteins were mainly apolipoproteins and mainly enriched in cholesterol metabolism-related pathways. Apolipoproteins family including APOA1, APOB, APOC1 were key proteins in protein-protein interaction network.Differentially expressed proteins in the exosomes may indicate the status of DR-TB from NDR-TB. Apolipoproteins family including APOA1, APOB, APOC1 may be involved in the pathogenesis of DR-TB by regulating cholesterol metabolism via exosomes.
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