西红花酸
活性氧
化学
细胞生物学
程序性细胞死亡
细胞凋亡
细胞损伤
氧化应激
线粒体
分子生物学
生物化学
生物
类胡萝卜素
作者
Wataru Otsu,Tomohiro Yako,Emi Sugisawa,Shinsuke Nakamura,Hideshi Tsusaki,Naofumi Umigai,Masamitsu Shimazawa,Hideaki Hara
标识
DOI:10.1016/j.jphs.2022.10.005
摘要
The corneal epithelium is located at the outermost layer of the ocular surface and continuously exposed to environmental factors, such as ultraviolet (UV) radiation from sunlight. UV irradiation causes excessive production of reactive oxygen species (ROS) in cells, which results in oxidative damage to membrane-bound organelles such as mitochondria, eventually leading to cell death. Crocetin, a natural carotenoid found in plants, has various biological properties including antioxidant activity. In this study, we investigated the effects of crocetin on UV-A-induced cell injury in the corneal epithelium. Using an in vitro system with the human corneal epithelial cell-transformed (HCE-T) cell line, pretreatment with 10 μM crocetin suppressed the reduction of cell viability induced by UV-A exposure. Crocetin ameliorated the decrease in oxygen consumption rates and the mitochondrial fragmentation that occurred following UV-A irradiation. Crocetin inhibited both ROS production and the activation of the apoptosis pathway; it also preserved the defects of epithelial cell polarity and barrier function in UV-A-irradiated HCE-T cells. The reduction in apical Mucin-16 expression was partially recovered in the presence of crocetin. Taking these findings together, we conclude that crocetin has a protective effect against UV-A irradiation-induced mitochondrial injury in corneal epithelial cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI