老化
转录组
衰老
基因敲除
下调和上调
生物标志物
皮肤老化
生物
计算生物学
细胞生物学
癌症研究
生物信息学
基因表达
医学
基因
生物化学
遗传学
皮肤病科
作者
Xiaozhen Peng,Yun Zhong,Rui Mao,Fanping He,Yu‐Fan Cheng,Mengting Chen,Lei Zhou,Hongfu Xie,Ji Li,Yiya Zhang
摘要
Abstract Ageing is an inevitable biological process characterized by progressive decline in physiological functions. It is a complex natural phenomenon that will cause structural and functional decline. Despite substantial progress in understanding the mechanism of ageing, both predictive biomarkers and preventive therapies remain limited. Using Weighted Gene Co‐expression Network Analysis (WGCNA) and machine learning techniques, we identified Carboxypeptidase E (CPE) as a pivotal marker of skin ageing, based on ageing‐related bulk transcriptome and single‐cell transcriptome data. Next, our investigation reveals downregulation of CPE in replicative, UVA‐induced, and H 2 O 2 ‐induced senescent human dermal fibroblast cells (HDFs). Furthermore, shRNA‐mediated CPE knockdown induced HDFs senescence, and overexpression of CPE delayed HDFs senescence. Moreover, downregulated CPE inhibits collagen synthesis and induces inflammation, highlighting its potential as a therapeutic target for skin ageing. In conclusion, our study demonstrated that CPE functions as a predictor and optional target for therapeutic intervention of skin ageing.
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