血管免疫母细胞性T细胞淋巴瘤
免疫球蛋白D
B细胞
流式细胞术
CD8型
免疫分型
病理
淋巴瘤
生物
淋巴细胞
CXCL13型
外周血单个核细胞
抗体
免疫学
T细胞
医学
免疫系统
趋化因子
体外
生物化学
趋化因子受体
作者
Hung-Lin Liu,Chun‐Kai Liao,Shao‐Wen Weng,Lee‐Yung Shih,Chih-Chi Chou,Huey-Ling You,Ming-Chung Wang,Wan‐Ting Huang
出处
期刊:Pathobiology
[S. Karger AG]
日期:2022-11-24
卷期号:90 (3): 187-198
被引量:2
摘要
Angioimmunoblastic T-cell lymphoma (AITL) is an aggressive T-cell lymphoma commonly associated with B-cell dysregulation. Correlations involving B-cell dysregulation and clinicopathological features remain unclear.We prospectively collected blood samples from 11 AITL patients and 17 healthy controls. The percentages of B-cell subpopulations and lymphocytes with IL-21 production were assessed using flow cytometry. Peripheral blood lymphocyte morphology was evaluated microscopically.Six of 11 (54.5%) patients presented with polyclonal hypergammaglobulinemia. Three of 11 (27.3%) tumor biopsies showed monoclonal immunoglobulin gene rearrangement. The patients exhibited significantly lower levels of naive (p < 0.001) and class-switched (p < 0.001) B cells than controls. The percentages of IgD-CD27- B cells (p = 0.007) and antibody-secreting cells (ASCs) (p = 0.001) were increased. Blood smears revealed atypical lymphocytes and immature plasma cells with morphological diversity. In comparison to normal controls, IL-21 production significantly increased in CD4+ (p < 0.001) and CD8+ (p = 0.020) T cells. B-cell clonality, RHOA G17V mutation, and the presence of sheets of clear cells and immature/mature plasma cells in lymph nodes were significantly associated with percentages of class-switched B cells and ASCs. The patients with circulating EBV DNA had a lower percentage of naive B cells (p = 0.009).Our results demonstrated a wide spectrum of peripheral B-cell morphologies and immunophenotypes of peripheral B cells in AITL. These findings correspond to dysregulated B-cell immunity and heterogeneous clinicopathological features.
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