TCEP
抗坏血酸
脱氢抗坏血酸
异质结
磷化氢
材料科学
化学
特里斯
X射线光电子能谱
光电子学
化学工程
无机化学
氧化还原
催化作用
生物化学
食品科学
工程类
作者
Jun‐Tao Cao,Bing Wang,Yu-Xiang Dong,Qian Wang,Shu‐Wei Ren,Yan‐Ming Liu,Weiwei Zhao
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2018-05-31
卷期号:3 (6): 1087-1092
被引量:71
标识
DOI:10.1021/acssensors.8b00332
摘要
This work reports the elegant bridging of enzymatic generation of electron donor with photogenerated hole-induced chemical redox cycling amplification (RCA) for innovative photoelectrochemical (PEC) immunoassay, by the aid of a heterojunction photoelectrode with split-type strategy. Specifically, the system was exemplified by the alkaline phosphatase (ALP) catalytic generation of ascorbic acid (AA), the redox cycling of AA by tris (2-carboxyethyl) phosphine (TCEP) as reductant, and the use of a novel Bi2S3/Bi2Sn2O7 heterojunction and myoglobin (Myo) as the photoelectrode and the target, respectively. After the immunoreaction and ALP-induced production of AA, the subsequent oxidation of AA by the photogenerated holes of the Bi2S3/Bi2Sn2O7 heterojunction could be cycled via the regeneration of AA by TCEP from the oxidized product of dehydroascorbic acid, leading to easy signal amplification for the sensitive immunoassay of Myo in real samples. It is believed that this work provided a basis for further design and development of general RCA-based PEC immunoassays.
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