A Specific Enzyme-Linked Immunosorbent Assay for Measuring β-Amyloid Protein Oligomers in Human Plasma and Brain Tissue of Patients With Alzheimer Disease

单体 阿尔茨海默病 脑组织 抗体 体内 化学 二聚体 β淀粉样蛋白 淀粉样蛋白(真菌学) 医学 病理 分子生物学 疾病 内科学 生物化学 免疫学 生物 遗传学 有机化学 聚合物
作者
Weiming Xia,Ting Yang,Ganesh M. Shankar,Imelda M. Smith,Yong Shen,Dominic M. Walsh,Dennis J. Selkoe
出处
期刊:Archives of neurology [American Medical Association]
卷期号:66 (2) 被引量:208
标识
DOI:10.1001/archneurol.2008.565
摘要

Objective: To examine in vivo levels of ␤-amyloid (A␤) oligomers (oA␤) vs monomeric A␤ in plasma and brain tissue of patients with sporadic and familial Alzheimer disease (AD) using a new enzyme-linked immunosorbent assay (ELISA) specific for oA␤.Design: To establish the oA␤ ELISA, the same Nterminal A␤ antibody was used for antigen capture and detection.Plasma and postmortem brain tissue from patients with AD and control subjects were systematically analyzed by conventional monomeric A␤ and new oA␤ ELISAs. Subjects:We measured oA␤ species in plasma samples from 36 patients with clinically well-characterized AD and 10 control subjects.In addition, postmortem samples were obtained from brain autopsies of 9 patients with verified AD and 7 control subjects.Main Outcome Measures: Oligomeric A␤ and 4 monomeric A␤ species in plasma samples from patients with AD and control subjects were measured by ELISA. Results:The specificity of the oA␤ ELISA was validated with a disulfide-crossed-linked, synthetic A␤ 1-40 Ser26Cys dimer that was specifically detected before but not after the dissociation of the dimers in ␤-mercaptoethanol.Plasma assays showed that relative oA␤ levels were closely associated with relative A␤ 42 monomer levels across all of the subjects.Analysis of sequential plasma samples from a subset of the patients with AD, including a patient with AD caused by a presenilin mutation, revealed decreases in both oA␤ and A␤ 42 monomer levels over a 1-to 2-year period.In brain tissue from 9 patients with AD and 7 control subjects, both oA␤ and monomeric A␤ 42 levels were consistently higher in the AD cases.Conclusions: An oA␤-specific ELISA reveals a tight link between oA␤ and A␤ 42 monomer levels in plasma and brain.Both forms can decline over time in plasma, presumably reflecting their increasing insolubility in the brain.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
gogoyoco发布了新的文献求助10
1秒前
xn201120发布了新的文献求助10
1秒前
英俊的铭应助西门访天采纳,获得30
2秒前
H2CO3发布了新的文献求助10
4秒前
4秒前
5秒前
5秒前
幸福大白发布了新的文献求助10
5秒前
爆米花应助Leoniko采纳,获得10
5秒前
5秒前
5秒前
李星完成签到,获得积分20
5秒前
6秒前
7秒前
7秒前
8秒前
赘婿应助YJ888采纳,获得10
9秒前
surong发布了新的文献求助10
10秒前
Shelby发布了新的文献求助10
10秒前
dyc238100发布了新的文献求助10
11秒前
树精发布了新的文献求助10
11秒前
liii发布了新的文献求助10
11秒前
11秒前
火柴盒完成签到,获得积分10
12秒前
如此这般发布了新的文献求助10
12秒前
13秒前
13秒前
14秒前
幸福大白发布了新的文献求助30
15秒前
Akitten关注了科研通微信公众号
15秒前
15秒前
闾丘剑封发布了新的文献求助10
17秒前
天天快乐应助Shelby采纳,获得10
17秒前
17秒前
华仔应助xn201120采纳,获得10
18秒前
木子李发布了新的文献求助10
18秒前
搬砖小土妞完成签到,获得积分20
19秒前
李星发布了新的文献求助10
20秒前
21秒前
21秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989510
求助须知:如何正确求助?哪些是违规求助? 3531756
关于积分的说明 11254536
捐赠科研通 3270255
什么是DOI,文献DOI怎么找? 1804947
邀请新用户注册赠送积分活动 882113
科研通“疑难数据库(出版商)”最低求助积分说明 809176