爪蟾
电压钳
细胞生物学
生物
异源表达
运输机
异源的
重组DNA
计算生物学
膜电位
生物化学
基因
作者
Manan Bhatt,Angela Di Iacovo,Tiziana Romanazzi,Cristina Roseti,Raffaella Cinquetti,Elena Bossi
出处
期刊:Membranes
[MDPI AG]
日期:2022-09-25
卷期号:12 (10): 927-927
被引量:13
标识
DOI:10.3390/membranes12100927
摘要
After 50 years, the heterologous expression of proteins in Xenopus laevis oocytes is still essential in many research fields. New approaches and revised protocols, but also classical methods, such as the two-electrode voltage clamp, are applied in studying membrane transporters. New and old methods for investigating the activity and the expression of Solute Carriers (SLC) are reviewed, and the kinds of experiment that are still useful to perform with this kind of cell are reported. Xenopus laevis oocytes at the full-grown stage have a highly efficient biosynthetic apparatus that correctly targets functional proteins at the defined compartment. This small protein factory can produce, fold, and localize almost any kind of wild-type or recombinant protein; some tricks are required to obtain high expression and to verify the functionality. The methodologies examined here are mainly related to research in the field of membrane transporters. This work is certainly not exhaustive; it has been carried out to be helpful to researchers who want to quickly find suggestions and detailed indications when investigating the functionality and expression of the different members of the solute carrier families.
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