依托三酯
色谱法
生物等效性
选择性反应监测
化学
蛋白质沉淀
电喷雾电离
串联质谱法
质谱法
电喷雾
高效液相色谱法
药代动力学
药理学
医学
有机化学
作者
Gabriel Onn Kit Loh,Emily Yii Ling Wong,Yvonne Tze Fung Tan,Siew Chyee Heng,Mardiana Saaid,Kit Yee Cheah,Nurul Diyana Mohd Sali,Nair Damenthi,Sharon Shi Min Ng,Long Chiau Ming,Kok Khiang Peh
出处
期刊:Molecules
[MDPI AG]
日期:2022-09-04
卷期号:27 (17): 5706-5706
被引量:3
标识
DOI:10.3390/molecules27175706
摘要
Etoricoxib is a non-steroidal anti-inflammatory drug (NSAID) used to treat pain and inflammation. The objective of the current study was to develop a sensitive, fast and high-throughput HPLC-ESI-MS/MS method to measure etoricoxib levels in human plasma using a one-step methanol protein precipitation technique. A tandem mass spectrometer equipped with an electrospray ionization (ESI) source operated in a positive mode and multiple reaction monitoring (MRM) were used for data collection. The quantitative MRM transition ions were m/z 359.15 > 279.10 and m/z 363.10 > 282.10 for etoricoxib and IS. The linear range was from 10.00 to 4000.39 ng/mL and the validation parameters were within the acceptance limits of the European Medicine Agency (EMA) and Food and Drug Analysis (FDA) guidelines. The present method was sensitive (10.00 ng/mL with S/N > 40), simple, selective (K prime > 2), and fast (short run time of 2 min), with negligible matrix effect and consistent recovery, suitable for high throughput analysis. The method was used to quantitate etoricoxib plasma concentrations in a bioequivalence study of two 120 mg etoricoxib formulations. Incurred sample reanalysis results further supported that the method was robust and reproducible.
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