荧光
葡萄糖氧化酶
检出限
猝灭(荧光)
选择性
纳米材料
化学
碳纤维
聚乙烯亚胺
材料科学
热液循环
光化学
催化作用
纳米技术
生物传感器
色谱法
化学工程
有机化学
生物化学
物理
量子力学
转染
复合材料
复合数
工程类
基因
作者
Yuxuan Hu,Jialin Wen,Dan Li,Yuting Li,Muidh Alheshibri,Minmin Zhang,Lingling Shui,Na Li
标识
DOI:10.1016/j.saa.2023.123149
摘要
In this work, a novel "turn-on" fluorescence sensor for the detection of H2O2 and glucose was developed based on green fluorescent carbon dots (CDs). The CDs was newly prepared by a facile one-pot hydrothermal method with Eosin Y and branched polyethylenimine as precursors. Interestingly, in the presence of H2O2 and HRP, the fluorescence of the CDs enhanced significantly with a red-shift emission due to their "aggregation". Meanwhile, the oxidation of glucose catalyzed by glucose oxidase could generate H2O2. Thus, a simple sensing system based on the CDs as fluorescent probes was constructed for H2O2 and glucose determination, avoiding the fluorescence quenching and subsequent recovery process in conventional turn-on strategy. The method showed good selectivity and sensitivity for glucose sensing with the detection limit of 0.12 μM. The method was further applied to glucose detection in real samples. The obtained results demonstrated the simplicity, selectivity and practicality of the method. This work expands the carbon nanomaterials with fluorescence emission enhancement properties. It provides a new and direct "turn-on" strategy for H2O2 and glucose detection, which could be a simple and effective tool for screening biological substances involved in H2O2-generation reaction.
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