Strategies to Increase the Production of Triterpene Acids in Ligzhi or Reishi Medicinal Mushroom (Ganoderma lucidum, Agaricomycetes): A Review

Ganoderic acids (GAs) are the main active ingredient of <i>Ganoderma lucidum</i>, which has been widely accepted as a medicinal mushroom. Due to the low yield of GAs produced by liquid cultured <i>Ganoderma</i> mycelium and solid cultured fruiting bodies, the commercial production and clinical application of GAs are limited. Therefore, it is important to increase the yield of GA in <i>G. lucidum</i>. A comprehensive literature search was performed with no set data range using the following keywords such as "triterpene," "ganoderic acids," "<i>Ganoderma lucidum</i>," and "Lingzhi" within the main databases including Web of Science, PubMed, and China National Knowledge Infrastructure (CNKI). The data were screened using titles and abstracts and those relevant to the topic were included in the paper and was not limited to studies published in English. Present review focuses on the four aspects: fermentation conditions and substrate, extrinsic elicitor, genetic engineering, and mutagenesis, which play significant roles in increasing triterpene acids production, thus providing an available reference for further research on <i>G. lucidum</i> fermentation.