Inhibition of autophagy mitigates cell migration and invasion in thyroid cancer

甲状腺间变性癌 自噬 医学 下调和上调 CD44细胞 癌症研究 甲状腺癌 癌症干细胞 死孢子体1 细胞培养 滤泡状甲状腺癌 癌症 癌细胞 细胞生物学 细胞 细胞凋亡 细胞迁移 生物 内科学 甲状腺乳突癌 生物化学 基因 遗传学
作者
Tammy M. Holm,Z. Christine Bian,Kanakaraju Marumudi,Jun‐Lin Guan
出处
期刊:Surgery [Elsevier BV]
卷期号:171 (1): 235-244 被引量:8
标识
DOI:10.1016/j.surg.2021.08.024
摘要

Autophagy is a highly conserved process for maintaining cellular homeostasis. Upregulation of autophagy promotes metastasis by promoting the cancer stem cell state while also stimulating tumor cell migration and invasion. We hypothesized that autophagy upregulation would be critical for cancer stem cell maintenance as well as cellular migration and invasion in thyroid cancer.Validated papillary (MDA-T32, MDA-T68), follicular (FTC-133), and anaplastic (ATC-8505c) human thyroid cancer cell lines in culture were first assessed for autophagic capacity after bafilomycin clamping. Cancer stem cells were quantified by flow cytometry for aldehyde dehydrogenase and thyrosphere formation assay. Scratch migration and Matrigel invasion assays were performed in the presence of known autophagy inhibitors: Lys05, chloroquine, and FIP200siRNA.Autophagy activity was observed across all cell lines. Thyrosphere formation, aldehyde dehydrogenase activity, and CD44 expression were reduced with inhibition of autophagy in MDA-T32, MDA-T68, FTC-133, and 8505c cells. Similarly, cell migration and invasion were attenuated: 42% (FIP200siRNA), 78% (Lys05), P < .001 in MDA-T32 cells; 54% (FIP200siRNA), 67% (Lys05), P < .001 in MDA-T68 cells; 73% (FIP200siRNA), 71% (Lys05), P < .001) in FTC-133 cells; and 60% (FIP200siRNA), 90% (Lys05), P < .001 in 8505c cells. Invasion assays demonstrated a 73%, 39%, 75%, and 65.1% reduction in the presence of Lys05 in T32, T68, FTC-133, and 8505c cells, respectively. We observed similar reductions in invasion with FIP200siRNA: 61%, 62%, 55%, and 81.4% in T32, T68, FTC-133, and 8505c cells.Autophagy is upregulated across multiple thyroid cancer subtypes. In thyroid cancer cell lines, inhibition of autophagy attenuates cancer stem cell viability, cell migration, and invasion suggesting a role for autophagy in the progression of thyroid cancer. Greater understanding of autophagy regulation in thyroid cancer will aid in developing targeted therapeutics.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
RoKing完成签到,获得积分10
2秒前
June发布了新的文献求助10
2秒前
2秒前
3秒前
3秒前
wanci应助搬砖打工人采纳,获得10
3秒前
量子星尘发布了新的文献求助10
4秒前
nini完成签到,获得积分10
5秒前
8秒前
nini发布了新的文献求助10
8秒前
希望天下0贩的0应助gwff采纳,获得10
9秒前
8R60d8应助科研通管家采纳,获得10
9秒前
8R60d8应助科研通管家采纳,获得10
9秒前
8R60d8应助科研通管家采纳,获得10
9秒前
斯文败类应助科研通管家采纳,获得10
9秒前
8R60d8应助科研通管家采纳,获得10
9秒前
8R60d8应助科研通管家采纳,获得10
9秒前
orixero应助科研通管家采纳,获得10
9秒前
10秒前
8R60d8应助科研通管家采纳,获得10
10秒前
寒冰寒冰完成签到,获得积分10
10秒前
共享精神应助科研通管家采纳,获得30
10秒前
所所应助XBP采纳,获得10
10秒前
大模型应助酷酷的麦片采纳,获得10
10秒前
11秒前
luowen完成签到,获得积分10
11秒前
12秒前
西红柿发布了新的文献求助10
13秒前
丘比特应助xiaozhouning采纳,获得30
13秒前
13秒前
乖猫要努力应助元谷雪采纳,获得10
13秒前
ding应助Psycho采纳,获得10
13秒前
思源应助xiaodong采纳,获得10
14秒前
智慧莎完成签到,获得积分10
15秒前
15秒前
15秒前
17秒前
nature完成签到 ,获得积分10
17秒前
17秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971333
求助须知:如何正确求助?哪些是违规求助? 3516028
关于积分的说明 11180607
捐赠科研通 3251147
什么是DOI,文献DOI怎么找? 1795693
邀请新用户注册赠送积分活动 875999
科研通“疑难数据库(出版商)”最低求助积分说明 805228