Advanced assisted reproductive technology in cattle: OPU-IVF review

India ranked 1 st in cow and buffalo population in the world with annual production of 187.75 MT of milk and 8.11 MT of meat, livestock contributes 4.1% in of total GDP.Even though, production is inadequate to fulfill the nutritional demands of population of the country.To generate bulk quantity of food products to fulfill the demands required to adopt new technologies or refining of the technologies, which in turn can increase in production and here, assisted reproductive technology (ART) can play a major role including In vitro fertilization (IVF) technology.In world, 6,66,000 embryos produced by IVF in 2016 and in Brazil IVP increased by 184.0% between 2005 and 2016.Live offspring of 25 species already been achieved though embryo transfer technology.Initially, in vitro embryo production (IVEP), the oocytes were collected from the slaughterhouse ovaries and at present, oocytes are being collected from the live animal by using OPU methodology.To maintain optimum quality of embryo production and production rate clean room preparations is radically important.Polluted air and volatile organic compounds are contributing in poor in vitro embryo production.IVF is become a regular tool to produce embryos for different purpose including research throughout the world.This technology needs further refinement and here we reviewed about Ovum pick up -In vitro fertilization (OPU-IVF) technique for cattle, clean room methodologies and pointed out the major considerations that can definitely help researchers.