Circ_0004491 stimulates guanine nucleotide-binding protein alpha subunit to inhibit the malignant progression of oral squamous cell carcinoma by sponging miR-2278

GNAS复合轨迹 基因敲除 癌症研究 细胞生长 分子生物学 生物 细胞凋亡 流式细胞术 基因 生物化学
作者
Tao Cheng,F. Huang,Yin Zhang,Zhen Zhou
出处
期刊:Journal of Dental Sciences [Elsevier BV]
卷期号:18 (1): 237-247
标识
DOI:10.1016/j.jds.2022.05.018
摘要

Circular RNA origin recognition complex subunit 4 (circORC4; ID: hsa_circ_0004491) have been confirmed to be a novel potential biomarker of oral squamous cell carcinoma (OSCC). This study aimed to explore the molecular mechanism of circ_0004491 in OSCC progression.Levels of circ_0004491, microRNA (miR)-2278, guanine nucleotide-binding protein alpha subunit (GNAS), Bax, Bcl-2, E-cadherin and ki-67 were detected by quantitative real-time PCR, western blotting and immunohistochemistry. The proliferation of OSCC cells was measured using colony formation assay and EdU staining. Cell apoptosis and motility were detected by flow cytometry and transwell assays respectively. Interaction between miR-2278 and circ_0004491 or GNAS was predicted by bioinformatics analysis and confirmed via luciferase reporter assay and RNA immunoprecipitation assay. Xenograft tumor model was used to analyze the role of circ_0004491 in tumor growth in vivo.Circ_0004491 was downregulated in OSCC tissues and cell lines. Circ_0004491 overexpression suppressed the proliferation, migration and invasion whereas facilitated the apoptosis of OSCC cells. Circ_0004491 acted as a molecular sponge for miR-2278, and circ_0004491 overexpression-mediated effect was partly reversed by miR-2278 mimic in OSCC cells. MiR-2278 interacted with the 3'UTR of GNAS. Circ_0004491 contributed to GNAS level by sponging miR-2278 in OSCC cells. GNAS knockdown restored miR-2278 inhibitor-mediated effect in OSCC cells. Circ_0004491 overexpression repressed xenograft tumor growth in vivo.Circ_0004491 can repress OSCC progression by regulation of miR-2278/GNAS axis, providing a possible circRNA-targeted therapy for OSCC.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
情怀应助无奈的白云采纳,获得10
1秒前
1秒前
乖猫要努力应助123456采纳,获得10
2秒前
2秒前
汉堡包应助巡音幻夜采纳,获得10
3秒前
3秒前
dicc发布了新的文献求助10
3秒前
元g发布了新的文献求助10
5秒前
5秒前
陈住气发布了新的文献求助10
6秒前
孙成成发布了新的文献求助10
6秒前
zyq完成签到,获得积分10
7秒前
7秒前
7秒前
友好驳发布了新的文献求助10
8秒前
8秒前
8秒前
10秒前
Sssssss完成签到 ,获得积分10
11秒前
cyy1226发布了新的文献求助10
11秒前
上官若男应助ljw采纳,获得10
13秒前
量子星尘发布了新的文献求助10
13秒前
陈住气完成签到,获得积分20
13秒前
14秒前
ww完成签到,获得积分10
14秒前
14秒前
蓝胖胖蓝完成签到,获得积分10
15秒前
苏航发布了新的文献求助10
17秒前
17秒前
海东来发布了新的文献求助30
17秒前
无花果应助爱撒娇的紫菜采纳,获得10
18秒前
18秒前
19秒前
大气的枫发布了新的文献求助10
20秒前
上官若男应助小西贝采纳,获得10
20秒前
22秒前
在水一方应助liuxuying采纳,获得10
23秒前
24秒前
巡音幻夜发布了新的文献求助10
24秒前
小郭完成签到,获得积分10
25秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975458
求助须知:如何正确求助?哪些是违规求助? 3519866
关于积分的说明 11199996
捐赠科研通 3256213
什么是DOI,文献DOI怎么找? 1798133
邀请新用户注册赠送积分活动 877386
科研通“疑难数据库(出版商)”最低求助积分说明 806305