How reliable is chlorophyll-a as algae proxy in lake environments? New insights from the perspective of n-alkanes.

Chlorophyll-a (Chl-a) has been employed as the "golden proxy" of algae biomass and algae cell densities in lake environments for many years. However, how reliable Chl-a is as algae proxy in lake environments needs further evaluation. Here, we take the eutrophic Lake Chaohu and 46 lakes and reservoirs across China as objects on temporal and spatial scales, respectively, to resolve this issue from the perspective of n-alkanes. Our results showed that Chl-a ranged from 10.5 to 735 μg∙L-1 with a geometric mean of 92.4 μg∙L-1 in Lake Chaohu. There were no statistically significant correlations between Chl-a and algae cell densities in all seasons (Pearson's correlation, p > 0.05), and also for macrophytes and terrestrial plants input (p > 0.05). It was related to the complex changes of environmental factors. By contrast, Chl-a ranged from 7.1 to 1608 μg∙L-1 with a geometric mean of 125 μg∙L-1 in nationwide lakes and reservoirs, and its occurrence was not only related to algae, but also associated with macrophytes and terrestrial plants (p < 0.05). In summary, Chl-a can be applied as an algae proxy, but its application is subject to certain restrictions. Besides, the multiple sources of Chl-a in lake environments may result in an overestimation of algae cell densities. Compared to Chl-a, biogenic n-heptadecane (bio C17) could be regarded as a potential alternative. Hence, we compared the advantages and disadvantages of bio C17 and Chl-a in the aspects of specificity, accuracy, sensitivity and applicability. We found that for most scenarios, their limitations could be surmounted by each other, but failed in some scenarios. Accordingly, an ensemble proxy system may be used for more reliable representation of algae in lake environments.