爬行
微流控
秀丽隐杆线虫
生化工程
计算机科学
纳米技术
工程类
生物
材料科学
生物化学
基因
解剖
作者
Qian Zhang,Jiadi Sun,Xiao Yuan,Xin Lü,Yongli Ye
标识
DOI:10.1016/j.trac.2021.116195
摘要
The nematode Caenorhabditis elegans (C. elegans) is characterized by small size, a transparent body, and a simple structure. However, traditional techniques (e.g., sorting and behavioral analysis) used in C. elegans behavior research are cumbersome; in addition, obtaining large amount of information from experiments is challenging. Microfluidic chips can process single or multiple nematodes in a high-throughput manner through their automation and miniaturization, providing a new platform for nematode research. This review mainly focuses on different stimuli, such as heavy metals, chemicals, drugs, neurotoxins, and pathogens. Based on behavioral indicators, such as crawling speed, swing frequency, and crawling methods, the applications of microfluidics in toxicity evaluation, drug screening, and aging development are summarized. Lastly, challenges and potential opportunities associated with the use of microfluidic technology for C. elegans research include evaluating the joint effect of mixed endogenous pollutants, establishing an integrated evaluation system, and building a big data platform.
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