牙周膜干细胞
衰老
细胞生物学
干细胞
维生素
维生素C
生物
牙周纤维
细胞培养
内科学
内分泌学
医学
生物化学
牙科
碱性磷酸酶
酶
遗传学
作者
Yongqiang Yang,Tao Wang,Sicheng Zhang,Sixun Jia,Hong Chen,Yufeng Duan,Shikai Wang,Guoqing Chen,Weidong Tian
摘要
Periodontal ligament stem cells (PDLSCs), as potential "seed cells" for periodontal tissue repair and regeneration, require to be expanded in vitro for a large scale. Senescence of PDLSCs occurred during long-term culture may compromise the therapeutic effects of PDLSCs. Medium supplements may be useful in antisenescence. However, the effects and mechanisms of vitamin C (Vc) treatment on PDLSCs during long-term culture are still unclear. In this study, we identified that Vc-treated PDLSCs cells maintained a slender morphology, higher growth rate and migration capacity, stemness, and osteogenic differentiation capability during a long-term culture. Moreover, we also identified that Notch3 was significantly upregulated during the cell senescence, and Vc treatment alleviated the senescence of PDLSCs through inhibition of Notch3 during long-term culture. In summary, Vc treatment suppressed PDLSCs senescence by reducing the expression of Notch3 and might be a simple and useful strategy to inhibit cellular senescence during the cell long-term culture.
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