神经突
PTEN公司
小发夹RNA
脊髓
脊髓损伤
皮质神经元
神经科学
医学
生物
细胞生物学
PI3K/AKT/mTOR通路
细胞凋亡
信号转导
基因敲除
体外
生物化学
作者
Hengxing Zhou,Xueying Li,Qiang Wu,Fuyuan Li,Zheng Fu,Chang Liu,Zhipin Liang,Tianci Chu,Tianyi Wang,Lu Lu,Guangzhi Ning,Xiaohong Kong,Shiqing Feng
出处
期刊:Regenerative Medicine
[Future Medicine]
日期:2014-12-15
卷期号:10 (4): 411-429
被引量:11
摘要
To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi.Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed.Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation.PTEN silencing may be promising for spinal cord injury repair.
科研通智能强力驱动
Strongly Powered by AbleSci AI