促炎细胞因子
上皮内淋巴细胞
免疫系统
失调
免疫学
免疫失调
肠道菌群
肠粘膜
固有层
生物
回肠
肠道通透性
炎症
内科学
上皮
医学
内分泌学
遗传学
作者
Leticia Muñoz,María‐José Borrero,Maria Cantera,Elisa Conde,Rosa del Campo,Macarena Rodríguez‐Serrano,M. Lario,Ana‐María Sánchez‐Díaz,Óscar Pastor,David Díaz,Laura García‐Bermejo,Jorge Monserrat,Melchor Álvarez‐Mon,Agustı́n Albillos
出处
期刊:Hepatology
[Wiley]
日期:2018-11-10
卷期号:70 (3): 925-938
被引量:92
摘要
In cirrhosis, intestinal dysbiosis, intestinal barrier impairment, and systemic immune system abnormalities lead to gut bacterial translocation (GBT) and bacterial infection. However, intestinal immune system dysfunction and its contribution to barrier damage are poorly understood. This study correlates immune system dysregulation in the intestines of rats at different stages of CCl 4 ‐induced cirrhosis with barrier function and pathogenic microbiota. The following variables were addressed in the small intestine: intraepithelial lymphocyte (IEL) and lamina propria lymphocyte (LPL) activation status and cytokine production (flow cytometry), cytokine mRNA and protein expression (quantitative real‐time PCR and immunofluorescence), microbiota composition of ileum content (16S recombinant DNA massive sequencing), permeability (fecal albumin loss), and epithelial junctions (immunohistochemistry and immunofluorescence). The intestinal mucosa in rats with cirrhosis showed a proinflammatory pattern of immune dysregulation in IELs and LPLs, which featured the expansion of activated lymphocytes, switch to a T helper 1 (Th1) regulatory pattern, and Th17 reduction. In rats with cirrhosis with ascites, this state was associated with epithelial junction protein disruption, fecal albumin loss, and GBT. Direct correlations ( P < 0.01) were observed between elevated interferon gamma (IFNγ)‐expressing T cytotoxic LPLs and fecal albumin and between inflammatory taxa abundance and IFNγ‐producing immune cells in the ileum. Bowel decontamination led to redistributed microbiota composition, reduced proinflammatory activation of mucosal immune cells, normalized fecal albumin levels, and diminished GBT; but there were no modifications in Th17 depletion. Conclusion : The intestinal mucosa of rats with cirrhosis acquires a proinflammatory profile of immune dysregulation that parallels the severity of cirrhosis; this impaired intestinal immune response is driven by gut dysbiosis and leads to disrupted barrier function, promoting GBT.
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