抗生素
肉汤微量稀释
抗菌剂
细菌
微生物学
孵化
细胞仪
生物
流式细胞术
最小抑制浓度
免疫学
遗传学
生物化学
作者
Daniel Spencer,Teagan F. Paton,Kieran T. Mulroney,Timothy J. J. Inglis,J. Mark Sutton,Hywel Morgan
标识
DOI:10.1038/s41467-020-18902-x
摘要
Abstract There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10 5 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.
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