肝细胞癌
体内
荧光
化学
双光子激发显微术
体外
转移
原位
癌细胞
生物物理学
血管生成
癌症研究
病理
癌症
生物化学
医学
生物
内科学
生物技术
有机化学
物理
量子力学
作者
Haidong Li,Yueqing Li,Qichao Yao,Jiangli Fan,Wen Sun,Saran Long,Kun Shao,Jianjun Du,Jingyun Wang,Xiaojun Peng
出处
期刊:Chemical Science
[The Royal Society of Chemistry]
日期:2018-11-27
卷期号:10 (6): 1619-1625
被引量:107
摘要
CD13/aminopeptidase N (APN), which is a zinc-dependent metalloproteinase, plays a vital role in the growth, migration, angiogenesis, and metastasis of tumours. Thus, in situ molecular imaging of endogenous APN levels is considerably significant for investigating APN and its different functions. In this study, a novel two-photon near-infrared (NIR) fluorescence probe DCM-APN was prepared to perform in vitro and in vivo tracking of APN. The N-terminal alanyl site of probe DCM-APN was accurately hydrolysed to the amino group, thereby liberating strong fluorescence owing to the recovery of the Intramolecular Charge Transfer (ICT) effect. By considering its outstanding selectivity, ultra-sensitivity (DL 0.25 ng mL-1) and favourable biocompatibility, the probe DCM-APN was used to distinguish between normal cells (LO2 cells) and cancer cells (HepG-2 and B16/BL6 cells). Furthermore, migration of hepatocellular carcinoma cells was apparently inhibited by ensuring that the APN catalytic cavity was occupied by bestatin. The identification of three-dimensional (3D) fluorescence in cancer tissues was completed under two-photon excitation coupled with lighting up hepatocellular carcinoma tumours in situ; this revealed that probe DCM-APN is an effective tool for detecting APN, thereby assisting in the early diagnosis of tumour in clinical medicine.
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