多糖
萃取(化学)
DPPH
化学
色谱法
抗氧化剂
响应面法
抗菌剂
生物化学
有机化学
作者
Jianhua Xie,Mingyue Shen,Mingyong Xie,Shaoping Nie,Yi Chen,Chang Li,Danfei Huang,Yuanxing Wang
标识
DOI:10.1016/j.carbpol.2012.02.068
摘要
Recently, renewed interest has grown in the use of ultrasonic-assisted extraction as an alternative approach to the traditional extraction methods. In this study, this novel extraction method was used to isolate polysaccharides from Cyclocarya paliurus (Batal.) Iljinskaja, and response surface methodology (RSM) was employed to optimize the extraction conditions. The optimal conditions for the extraction of polysaccharides were determined to be the ratio of liquid to solid of 8, extraction time of 59 min and extraction temperature of 58 °C. Under these optimal conditions, the yield of polysaccharides obtained was 4.91 ± 0.11%, which was well matched with the value predicted by the model. In vitro antioxidant assays showed that the polysaccharides exhibited strong DPPH radicals (92.09% at 0.25 mg/ml) and self-oxidation of 1,2,3-phentriol (37.22% at 1 mg/ml) scavenging activities, moderate hydroxyl radicals (43.18% at 1 mg/ml) scavenging activity and lipid peroxidation inhibitory effect (31.66% at 1 mg/ml). In addition, the polysaccharides showed moderate antimicrobial activity.
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