细胞生物学
癌症研究
蛋白激酶C
髓系白血病
化学
细胞分化
MAPK/ERK通路
蛋白激酶A
激酶
生物
生物化学
基因
作者
Xinghai Shen,Guo-Lin Xiong,Jie Yu,Xiaoqing He,Yu Cui,Yanfeng Zhang,Ya-Jun Shan,Shuang Xing,Meng Yang,Xiaolan Liu,Bo Dong,Lisheng Wang,Qing-Liang Luo,Zu-Yin Yu,Yang Cong
出处
期刊:Cancer Letters
[Elsevier]
日期:2015-01-01
卷期号:356 (2): 686-696
被引量:20
标识
DOI:10.1016/j.canlet.2014.10.018
摘要
As acute myeloid leukemia (AML) cells are characterized by uncontrolled self-renewal and impaired cellular differentiation, induction of terminal differentiation of leukemia cells by differentiating agents has been proposed as an attractive therapeutic strategy to treat AML. Here, we demonstrated that prostratin, a potent protein kinase C (PKC) activator, inhibited the growth of myeloid leukemia cells by a predominant G1 arrest with variable induction of apoptosis. Conversely, prostratin induced significant differentiation of AML cell lines and primary AML blasts as evidenced by morphology and immunophenotyping. The effects of prostratin were PKC dependent, and activation of mitogen-activated protein (MAP)/extracellular signal-regulated kinase (ERK) kinase (MEK) 1/2 by PKC was required for prostratin-induced cell differentiation. Consequently, prostratin reprogrammed transcriptional factor expression, and ectopic expression of c-Myc in HL-60 cells significantly eliminated prostratin-mediated cellular differentiation and cell cycle arrest, indicating an essential role for c-Myc suppression in the differentiation-inducing effects of prostratin. Finally, prostratin was able to potentiate cellular differentiation induced by chemotherapeutic agents such as Ara-C. Together, we proposed that prostratin alone or administered with other anticancer agents may be effective in differentiation therapy of AML.
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