已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Extracellular vesicle‐mediated delivery of circDYM alleviates CUS‐induced depressive‐like behaviours

化学 细胞生物学 微泡 外体 医学
作者
Xiaoyu Yu,Ying Bai,Bing Han,Minzi Ju,Tianci Tang,Ling Shen,Mingyue Li,Li Yang,Zhijun Zhang,Guoku Hu,Jie Chao,Yuan Zhang,Honghong Yao
出处
期刊:Journal of extracellular vesicles [Wiley]
卷期号:11 (1)
标识
DOI:10.1002/jev2.12185
摘要

Major depressive disorder (MDD) is the most prevalent psychiatric disorder worldwide and severely limits psychosocial function and quality of life, but no effective medication is currently available. Circular RNAs (circRNAs) have been revealed to participate in the MDD pathological process. Targeted delivery of circRNAs without blood-brain barrier (BBB) restriction for remission of MDD represents a promising approach for antidepressant therapy. In this study, RVG-circDYM-extracellular vesicles (RVG-circDYM-EVs) were engineered to target and preferentially transfer circDYM to the brain, and the effect on the pathological process in a chronic unpredictable stress (CUS) mouse model of depression was investigated. The results showed that RVG-circDYM-EVs were successfully purified by ultracentrifugation from overexpressed circDYM HEK 293T cells, and the characterization of RVG-circDYM-EVs was successfully demonstrated in terms of size, morphology and specific markers. Beyond demonstrating proof-of-concept for an RNA drug delivery technology, we observed that systemic administration of RVG-circDYM-EVs efficiently delivered circDYM to the brain, and alleviated CUS-induced depressive-like behaviours, and we discovered that RVG-circDYM-EVs notably inhibited microglial activation, BBB leakiness and peripheral immune cells infiltration, and attenuated astrocyte disfunction induced by CUS. CircDYM can bind mechanistically to the transcription factor TAF1 (TATA-box binding protein associated factor 1), resulting in the decreased expression of its downstream target genes with consequently suppressed neuroinflammation. Taken together, our findings suggest that extracellular vesicle-mediated delivery of circDYM is effective for MDD treatment and promising for clinical applications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
pasi完成签到,获得积分20
1秒前
平山小果子完成签到,获得积分10
3秒前
蓉蓉完成签到,获得积分10
4秒前
breeze完成签到,获得积分10
5秒前
英俊的铭应助sbmanishi采纳,获得10
6秒前
8秒前
9秒前
13秒前
科研小白发布了新的文献求助10
14秒前
结实的涵柏完成签到 ,获得积分10
15秒前
19秒前
timemaster666应助科研小白采纳,获得10
19秒前
zyx完成签到,获得积分10
20秒前
哈哈哈哈发布了新的文献求助10
22秒前
田様应助木木木扎特采纳,获得10
22秒前
故意的棒棒糖完成签到 ,获得积分10
24秒前
25秒前
科研通AI2S应助欢喜发卡采纳,获得10
27秒前
科研小白完成签到,获得积分20
30秒前
pasi发布了新的文献求助10
30秒前
乐乐乐乐乐乐应助feixiang采纳,获得10
33秒前
_hyl完成签到 ,获得积分10
35秒前
38秒前
39秒前
雷锋完成签到,获得积分10
39秒前
赴简发布了新的文献求助10
40秒前
手术刀完成签到 ,获得积分10
42秒前
44秒前
49秒前
zeice完成签到 ,获得积分10
52秒前
黯然完成签到 ,获得积分10
57秒前
58秒前
辛勤晓旋完成签到,获得积分10
59秒前
欣喜的代容完成签到 ,获得积分10
1分钟前
桐桐应助吃饭采纳,获得10
1分钟前
1分钟前
superbanggg完成签到,获得积分10
1分钟前
畅快的谷秋完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Foreign Policy of the French Second Empire: A Bibliography 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3146673
求助须知:如何正确求助?哪些是违规求助? 2797981
关于积分的说明 7826310
捐赠科研通 2454478
什么是DOI,文献DOI怎么找? 1306289
科研通“疑难数据库(出版商)”最低求助积分说明 627692
版权声明 601522