Genetic diversity and evolution of goose astrovirus in the east of China

Transboundary and Emerging DiseasesEarly View ORIGINAL ARTICLE Genetic diversity and evolution of goose astrovirus in the east of China Zhiguo Fei, Zhiguo Fei Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorAnqi Jiao, Anqi Jiao Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorMinli Xu, Minli Xu Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorJiaqiang Wu, Corresponding Author Jiaqiang Wu wujiaqiang2000@sina.com Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, China College of Life Sciences, Shandong Normal University, Jinan, Shandong, China Correspondence Lin Zhang, Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 202 Gongye North Road, Jinan, Shandong, China. Email: lzhang3406@163.com Jiaqiang Wu, [object HTMLSpanElement] Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 202 Gongye North Road, Jinan, Shandong, China. Email: wujiaqiang2000@sina.comSearch for more papers by this authorYu Wang, Yu Wang Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorJiang Yu, Jiang Yu orcid.org/0000-0002-7550-5041 Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorLu Lu, Lu Lu Emerging Economic Formats Research Institute, Shandong Management University, Jinan, Shandong, ChinaSearch for more papers by this authorWanchun Jiang, Wanchun Jiang College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, Hebei, ChinaSearch for more papers by this authorGuangwei Zhu, Guangwei Zhu School of Life Sciences, Qilu Normal University, Jinan, Shandong, ChinaSearch for more papers by this authorWenbo Sun, Wenbo Sun Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorZhi Chen, Zhi Chen Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorYuyu Zhang, Yuyu Zhang Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorSufang Ren, Sufang Ren Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorFei Liu, Fei Liu Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorLin Zhang, Corresponding Author Lin Zhang lzhang3406@163.com orcid.org/0000-0003-4763-8063 Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, China Correspondence Lin Zhang, Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 202 Gongye North Road, Jinan, Shandong, China. Email: lzhang3406@163.com Jiaqiang Wu, [object HTMLSpanElement] Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 202 Gongye North Road, Jinan, Shandong, China. Email: wujiaqiang2000@sina.comSearch for more papers by this author Zhiguo Fei, Zhiguo Fei Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorAnqi Jiao, Anqi Jiao Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorMinli Xu, Minli Xu Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorJiaqiang Wu, Corresponding Author Jiaqiang Wu wujiaqiang2000@sina.com Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, China College of Life Sciences, Shandong Normal University, Jinan, Shandong, China Correspondence Lin Zhang, Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 202 Gongye North Road, Jinan, Shandong, China. Email: lzhang3406@163.com Jiaqiang Wu, [object HTMLSpanElement] Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 202 Gongye North Road, Jinan, Shandong, China. Email: wujiaqiang2000@sina.comSearch for more papers by this authorYu Wang, Yu Wang Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorJiang Yu, Jiang Yu orcid.org/0000-0002-7550-5041 Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorLu Lu, Lu Lu Emerging Economic Formats Research Institute, Shandong Management University, Jinan, Shandong, ChinaSearch for more papers by this authorWanchun Jiang, Wanchun Jiang College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, Hebei, ChinaSearch for more papers by this authorGuangwei Zhu, Guangwei Zhu School of Life Sciences, Qilu Normal University, Jinan, Shandong, ChinaSearch for more papers by this authorWenbo Sun, Wenbo Sun Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorZhi Chen, Zhi Chen Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorYuyu Zhang, Yuyu Zhang Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorSufang Ren, Sufang Ren Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorFei Liu, Fei Liu Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, ChinaSearch for more papers by this authorLin Zhang, Corresponding Author Lin Zhang lzhang3406@163.com orcid.org/0000-0003-4763-8063 Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, Shandong, China Correspondence Lin Zhang, Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 202 Gongye North Road, Jinan, Shandong, China. Email: lzhang3406@163.com Jiaqiang Wu, [object HTMLSpanElement] Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, 202 Gongye North Road, Jinan, Shandong, China. Email: wujiaqiang2000@sina.comSearch for more papers by this author First published: 05 April 2022 https://doi.org/10.1111/tbed.14542 Zhiguo Fei and Anqi Jiao contributed equally to this work. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Goose astrovirus (GAstV), an agent of fatal visceral gout in goslings, has been widely circulating in eastern China since 2017, but little is known about its genetic diversity and systematic evolution. In this study, we isolated and sequenced nine nearly full-length GAstV genomes and conducted comprehensive genetic diversity and evolutionary analysis and compared them with other reported GAstV sequences. Our results indicated that two genotypic species of GAstV were circulating in China, and GAstV-2 subgenotype II-c had arisen as the dominant genotype in Shandong province and across the whole country. Multiple alignments of GAstV amino acid sequences revealed several characteristic mutations in GAstV-2 II-c strains, as well as additional residues in the nine new isolates which varied over time. Phylogenetic analysis of three open reading frames demonstrated different evolutionary histories. Evidence of natural recombination was also detected in GAstV, with most of the recombination occurring in the GAstV-2 II-c subgenotype. Molecular adaptation analyses revealed that the evolution of GAstV was shaped by strong negative selection, although a number of amino acids, which potentially affect host infection and cell entry, were subjected to positive pressure. Overall, these findings improve our understanding of the epidemiology and evolution of GAstV and may help in the development of vaccines and diagnostics. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available from the corresponding author upon reasonable request. Supporting Information Filename Description tbed14542-sup-0001-SuppMat.doc1.4 MB Supporting information. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. 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