单克隆抗体
化学
色谱法
回收率
分子生物学
抗体
生物
免疫学
作者
Huihui Wu,Xinxin Xu,Liqiang Liu,Liguang Xu,Hua Kuang,Chuanlai Xu
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2021-01-01
卷期号:146 (22): 6831-6839
被引量:11
摘要
In this study, a highly specific and sensitive monoclonal antibody (mAb) against quinclorac (Qui) was prepared. Based on the selected mAb, 2G3, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip assay were established for the detection of Qui in actual samples. The 50%-inhibitory concentration of mAb 2G3 against Qui was 48.763 ng mL-1. No cross-reaction with other quinolines indicated that mAb 2G3 had high specificity. The recovery of the established ic-ELISA method was in the range of 85.6% to 98.9%. The cut-off value of Qui in cucumber and tomato by immunochromatographic strip was 200 ng g-1. The analysis results of ic-ELISA and immunochromatographic strip assay were consistent with the results of LC-MS/MS, which further proved that the established ic-ELISA and immunochromatographic strip assay could provide valuable tools for the rapid detection of Qui residues in cucumber and tomato samples.
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