异弹目
代谢工程
发光杆菌属
生物反应器
辣椒疫霉
拉伤
生物
抗菌剂
细菌
合成生物学
产量(工程)
基因工程
生物技术
基因
微生物学
计算生物学
重组DNA
食品科学
植物
生物化学
胡椒粉
遗传学
材料科学
解剖
冶金
作者
Youcai Qin,Fenglian Jia,Xunhua Zheng,Xiaohui Li,Jiaqi Duan,Beibei Li,Hongfei Shen,Xiufen Yang,Jie Ren,Guangyue Li
标识
DOI:10.1021/acs.jafc.3c01793
摘要
Xenocoumacin 1 (Xcn1) is an excellent antimicrobial natural product against Phytophthora capsici. However, the commercial development of Xcn1 is hindered by the low yield, which results in high application costs. In this study, multiple metabolic strategies, including blocking the degradation pathway, promoter engineering, and deletion of competing biosynthetic gene clusters, were employed to improve the production of Xcn1, which was increased from 0.07 to 0.91 g/L. The formation of Xcn1 reached 1.94 g/L in the TB medium with the final strain T3 in a shake flask and further reached 3.52 g/L in a 5 L bioreactor, which is the highest yield ever reported. The engineered strain provides a valuable platform for production of Xcn1, and the possible commercial development of the biofungicide. We anticipate that the metabolic engineering strategies utilized in this study and the constructed constitutive promoter library can be widely applied to other bacteria of the genera Xenorhabdus and Photorhabdus.
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