下调和上调
卵泡期
滤泡囊肿
发病机制
细胞凋亡
生物
卵泡
内分泌学
内科学
ATG12
男科
自噬
细胞生物学
卵巢
基因
医学
免疫学
遗传学
ATG5型
作者
Maosheng Cao,Xue Chen,Yueying Wang,Lu Chen,Yun Zhao,Chunjin Li,Xu Zhou
摘要
Abstract Follicular cysts are a common reproductive disorder in domestic animals that cause considerable economic losses to the farming industry. Effective prevention and treatment methods are lacking because neither the pathogenesis nor formation mechanisms of follicular cysts are well‐understood. In this study, we first investigated the granulosa cells (GCs) of cystic follicles isolated from pigs. We observed a significant reduction in the expression of methyltransferase‐like 3 (METTL3). Subsequent experiments revealed that METTL3 downregulation in GCs caused a decrease in m 6 A modification of pri‐miR‐21. This reduction further inhibited DGCR8 recognition and binding to pri‐miR‐21, dampening the synthesis of mature miR‐21‐5p. Additionally, the decrease in miR‐21‐5p promotes IL‐1β expression in GCs. Elevated IL‐1β activates the NFκB pathway, in turn upregulating apoptotic genes TNFa and BAX / BCL2 . The subsequent apoptosis of GCs and inhibition of autophagy causes downregulation of CYP19A1 expression. These processes lower oestrogen secretion and contribute to follicular cyst formation. In conclusion, our findings provide a foundation for understanding and further exploring the mechanisms of follicular‐cyst development in farm animals. This work has important implications for treating ovarian disorders in livestock and could potentially be extended to humans.
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