The sphingolipids change in exosomes from cancer patients and association between exosome release and sphingolipids level based on a pseudotargeted lipidomics method

鞘脂 脂类学 微泡 鞘磷脂 外体 化学 ESCRT公司 生物发生 神经酰胺 癌症 细胞生物学 生物化学 小RNA 内科学 生物 胆固醇 内体 细胞内 基因 医学 细胞凋亡
作者
Kaige Yang,Wenchang Fu,Mengjiao Deng,Xinyan Li,Mingyuan Wu,Yan Wang
出处
期刊:Analytica Chimica Acta [Elsevier]
卷期号:1305: 342527-342527 被引量:2
标识
DOI:10.1016/j.aca.2024.342527
摘要

The lipid based ESCRT-independent mechanism, which contributes to MVB formation, is one of the crucial procedures in exosome biogenesis. n-SMase is a key lipid metabolism enzyme in this mechanism and can induce the hydrolysis of sphingomyelins (SMs) to ceramides (Cers), thereby promoting the formation of ILVs inside MVBs. Therefore, the regulation of n-SMase can realize the alteration in exosome release. According to the fact that cancer-associated cells have a tendency to release more exosomes than healthy cells, lipid extracts in exosomes from healthy volunteers, HCC and ICC patients were analyzed by a novel pseudotargeted lipidomics method focused on sphingolipids (SLs) to explore whether cancer-related features regulate the release of exosomes through the above pathway. Multivariate analysis based on the SLs expression could distinguish three groups well indicated that the SLs expression among the three groups were different. In cancer groups, two species of critical Cers were up-regulated, denoted as Cer (d18:1_16:0) and Cer (d18:1_18:0), while 55 kinds of SLs were down-regulated, including 40 species of SMs, such as SM (d18:1_16:0), SM (d18:1_18:1) and SM (d18:1_24:0). Meanwhile, several species of SM/Cer exhibited significant down-regulation. This substantial enhancement of the SMs hydrolysis to Cers process during exosome biogenesis suggested that cancer-related features may potentially promote an increase in exosome release through ESCRT-independent mechanism. Moreover, differential SLs have a capability of becoming potential biomarkers for disease diagnosis and classification with an AUC value of 0.9884 or 0.9806 for the comparison between healthy group and HCC or ICC groups, respectively. In addition, an association analysis conducted on the cell lines showed that changes in the SM/Cer contents in cells and their exosomes were negatively correlated with the levels of released exosomes, implied the regulation of exosome release levels can be achieved by modulating n-SMase and subsequent SL expression.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
小青新发布了新的文献求助20
1秒前
zy完成签到,获得积分10
3秒前
3秒前
谨慕轩发布了新的文献求助10
3秒前
靜心发布了新的文献求助10
4秒前
wlnhyF发布了新的文献求助10
7秒前
7秒前
稳如老狗发布了新的文献求助10
9秒前
一二完成签到,获得积分10
11秒前
不配.应助谨慕轩采纳,获得10
11秒前
王兴雨完成签到,获得积分10
13秒前
13秒前
16秒前
陈棋清发布了新的文献求助10
17秒前
善学以致用应助刘哔采纳,获得10
17秒前
17秒前
瘦瘦达完成签到,获得积分10
18秒前
18秒前
18秒前
科研通AI2S应助稳如老狗采纳,获得10
19秒前
弘木发布了新的文献求助10
19秒前
20秒前
collapsar1应助靜心采纳,获得10
22秒前
李大姐发布了新的文献求助10
23秒前
跳跃梦岚发布了新的文献求助20
24秒前
弘木完成签到,获得积分10
25秒前
25秒前
25秒前
哈利波特发布了新的文献求助10
25秒前
nns完成签到,获得积分10
27秒前
hqq2312发布了新的文献求助10
28秒前
顾北发布了新的文献求助10
28秒前
陈棋清完成签到,获得积分10
29秒前
liyingyan发布了新的文献求助30
29秒前
30秒前
wulalala完成签到,获得积分10
32秒前
行隐应助rossliyi采纳,获得10
34秒前
33097完成签到,获得积分10
36秒前
雪白问兰应助Yan采纳,获得10
37秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Le dégorgement réflexe des Acridiens 800
Defense against predation 800
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3136281
求助须知:如何正确求助?哪些是违规求助? 2787312
关于积分的说明 7780828
捐赠科研通 2443293
什么是DOI,文献DOI怎么找? 1299081
科研通“疑难数据库(出版商)”最低求助积分说明 625325
版权声明 600905