亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Petunia bZIP transcription factor PhbZIP3 is involved in floral volatile benzenoid/phenylpropanoid biosynthesis through regulating the expression of PhPAL2 and PhBSMT

牵牛花 生物 苯丙素 bZIP域 转录因子 基因 遗传学 发起人 生物合成 基因表达 亮氨酸拉链
作者
Jianxin Fu,Yu Guo,Hongsheng Qing,Xinyue Liu,Chao Zhang
出处
期刊:Scientia Horticulturae [Elsevier BV]
卷期号:326: 112719-112719
标识
DOI:10.1016/j.scienta.2023.112719
摘要

Floral volatile benzenoid/phenylpropanoid compounds (FVBPs) are essential plant secondary metabolites and play a pivotal role in plant reproduction and development. However, the knowledge of transcriptional regulation of its biosynthesis pathway remains limited. Although extensively implicated in the regulation of plant secondary metabolisms, the basic leucine zipper (bZIP) family has not been reported to regulate FVBP biosynthesis. This study aimed to screen out important bZIP transcription factor members in the transcriptional regulation of FVBP biosynthesis in petunia. The entirety of bZIP genes throughout the petunia genome was ascertained and in-depth investigations into their phylogenetic relationships, structural characteristics, expression patterns, and potential interactions with structural genes related to FVBP biosynthesis were conducted. Outcomes showed that 77 bZIP genes of petunia could be categorized into 11 groups. The significant quantitative differences of introns and conserved motifs among the petunia bZIP members, along with the variety of cis-acting elements, reflected the structure diversity of petunia bZIPs. Seven candidate bZIP genes for regulating FVBP biosynthesis were selected because of their up-regulated expression level after flower opening according to RNA sequencing data. Based on the qPCR and the subcellular localization analysis, one nuclear-localized member, PhbZIP3 was screened out. PhbZIP3 was mainly expressed in the corolla, and its expression level increased significantly during the anthesis period. Dual luciferase assay revealed that PhbZIP3 could enhance the expression of PhPAL2 and PhBSMT. The findings from the yeast one-hybrid assay further corroborated the interaction between PhbZIP3 and the promoters of PhPAL2 and PhBSMT. Taken together, these results suggest that PhbZIP3 play a pivotal role in the regulatory mechanism of FVBP biosynthesis in petunia.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
又来注水了完成签到,获得积分10
10秒前
快乐海豚完成签到 ,获得积分10
16秒前
科研通AI5应助科研通管家采纳,获得10
20秒前
科研狗的春天完成签到 ,获得积分10
23秒前
Lucas应助董可以采纳,获得10
30秒前
36秒前
Aqib发布了新的文献求助10
41秒前
42秒前
科研通AI5应助西瓜嘻嘻嘻采纳,获得10
45秒前
吴昕昕发布了新的文献求助10
47秒前
未晚完成签到 ,获得积分10
49秒前
香蕉觅云应助Aqib采纳,获得10
53秒前
早晨发布了新的文献求助10
59秒前
孤鸿.完成签到 ,获得积分10
1分钟前
1分钟前
彭于晏应助11采纳,获得10
1分钟前
1分钟前
吴昕昕完成签到,获得积分10
1分钟前
1分钟前
iwaking完成签到,获得积分10
1分钟前
hxn发布了新的文献求助30
1分钟前
通通发布了新的文献求助10
1分钟前
早晨完成签到,获得积分10
1分钟前
Lucas应助通通采纳,获得10
1分钟前
1分钟前
WANG完成签到,获得积分10
1分钟前
有机发布了新的文献求助10
1分钟前
摆渡人完成签到,获得积分10
1分钟前
提桶跑路完成签到 ,获得积分10
1分钟前
温柔的天奇完成签到 ,获得积分10
1分钟前
实验耗材完成签到 ,获得积分10
1分钟前
1分钟前
lhlhl完成签到,获得积分10
1分钟前
1分钟前
1分钟前
小小林发布了新的文献求助10
2分钟前
zl13332完成签到 ,获得积分10
2分钟前
2分钟前
通通完成签到 ,获得积分10
2分钟前
hhh完成签到 ,获得积分10
2分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990020
求助须知:如何正确求助?哪些是违规求助? 3532077
关于积分的说明 11256276
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805139
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228