Petunia bZIP transcription factor PhbZIP3 is involved in floral volatile benzenoid/phenylpropanoid biosynthesis through regulating the expression of PhPAL2 and PhBSMT

牵牛花 生物 苯丙素 bZIP域 转录因子 基因 遗传学 发起人 生物合成 基因表达 亮氨酸拉链
作者
Jianxin Fu,Yu Guo,Hongsheng Qing,Xinyue Liu,Chao Zhang
出处
期刊:Scientia Horticulturae [Elsevier BV]
卷期号:326: 112719-112719
标识
DOI:10.1016/j.scienta.2023.112719
摘要

Floral volatile benzenoid/phenylpropanoid compounds (FVBPs) are essential plant secondary metabolites and play a pivotal role in plant reproduction and development. However, the knowledge of transcriptional regulation of its biosynthesis pathway remains limited. Although extensively implicated in the regulation of plant secondary metabolisms, the basic leucine zipper (bZIP) family has not been reported to regulate FVBP biosynthesis. This study aimed to screen out important bZIP transcription factor members in the transcriptional regulation of FVBP biosynthesis in petunia. The entirety of bZIP genes throughout the petunia genome was ascertained and in-depth investigations into their phylogenetic relationships, structural characteristics, expression patterns, and potential interactions with structural genes related to FVBP biosynthesis were conducted. Outcomes showed that 77 bZIP genes of petunia could be categorized into 11 groups. The significant quantitative differences of introns and conserved motifs among the petunia bZIP members, along with the variety of cis-acting elements, reflected the structure diversity of petunia bZIPs. Seven candidate bZIP genes for regulating FVBP biosynthesis were selected because of their up-regulated expression level after flower opening according to RNA sequencing data. Based on the qPCR and the subcellular localization analysis, one nuclear-localized member, PhbZIP3 was screened out. PhbZIP3 was mainly expressed in the corolla, and its expression level increased significantly during the anthesis period. Dual luciferase assay revealed that PhbZIP3 could enhance the expression of PhPAL2 and PhBSMT. The findings from the yeast one-hybrid assay further corroborated the interaction between PhbZIP3 and the promoters of PhPAL2 and PhBSMT. Taken together, these results suggest that PhbZIP3 play a pivotal role in the regulatory mechanism of FVBP biosynthesis in petunia.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
6秒前
第三人称的自己完成签到,获得积分10
7秒前
12秒前
13秒前
14秒前
123完成签到,获得积分10
17秒前
18秒前
18秒前
执着又蓝发布了新的文献求助10
19秒前
19秒前
anna完成签到,获得积分10
21秒前
anna发布了新的文献求助10
24秒前
27秒前
Liufgui应助杜兰特工队采纳,获得10
28秒前
卿欣完成签到 ,获得积分10
29秒前
学术渣渣发布了新的文献求助10
32秒前
小情绪应助小刺猬采纳,获得10
32秒前
34秒前
34秒前
36秒前
Hathaway完成签到,获得积分10
38秒前
shuang0116发布了新的文献求助10
38秒前
吴所谓发布了新的文献求助50
39秒前
Liufgui应助听话的惜梦采纳,获得10
39秒前
充电宝应助影子采纳,获得10
40秒前
折耳根完成签到 ,获得积分10
40秒前
42秒前
犹豫的夏旋完成签到 ,获得积分10
42秒前
44秒前
46秒前
47秒前
量子星尘发布了新的文献求助10
48秒前
汉堡包应助Dmooou采纳,获得10
48秒前
今后应助MingqingFang采纳,获得10
52秒前
52秒前
jungle完成签到 ,获得积分10
52秒前
去追完成签到 ,获得积分10
52秒前
Cloud完成签到 ,获得积分10
52秒前
影子发布了新的文献求助10
54秒前
55秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988997
求助须知:如何正确求助?哪些是违规求助? 3531351
关于积分的说明 11253520
捐赠科研通 3269928
什么是DOI,文献DOI怎么找? 1804830
邀请新用户注册赠送积分活动 882063
科研通“疑难数据库(出版商)”最低求助积分说明 809068