Petunia bZIP transcription factor PhbZIP3 is involved in floral volatile benzenoid/phenylpropanoid biosynthesis through regulating the expression of PhPAL2 and PhBSMT

牵牛花 生物 苯丙素 bZIP域 转录因子 基因 遗传学 发起人 生物合成 基因表达 亮氨酸拉链
作者
Jianxin Fu,Yu Guo,Hongsheng Qing,Xinyue Liu,Chao Zhang
出处
期刊:Scientia Horticulturae [Elsevier BV]
卷期号:326: 112719-112719
标识
DOI:10.1016/j.scienta.2023.112719
摘要

Floral volatile benzenoid/phenylpropanoid compounds (FVBPs) are essential plant secondary metabolites and play a pivotal role in plant reproduction and development. However, the knowledge of transcriptional regulation of its biosynthesis pathway remains limited. Although extensively implicated in the regulation of plant secondary metabolisms, the basic leucine zipper (bZIP) family has not been reported to regulate FVBP biosynthesis. This study aimed to screen out important bZIP transcription factor members in the transcriptional regulation of FVBP biosynthesis in petunia. The entirety of bZIP genes throughout the petunia genome was ascertained and in-depth investigations into their phylogenetic relationships, structural characteristics, expression patterns, and potential interactions with structural genes related to FVBP biosynthesis were conducted. Outcomes showed that 77 bZIP genes of petunia could be categorized into 11 groups. The significant quantitative differences of introns and conserved motifs among the petunia bZIP members, along with the variety of cis-acting elements, reflected the structure diversity of petunia bZIPs. Seven candidate bZIP genes for regulating FVBP biosynthesis were selected because of their up-regulated expression level after flower opening according to RNA sequencing data. Based on the qPCR and the subcellular localization analysis, one nuclear-localized member, PhbZIP3 was screened out. PhbZIP3 was mainly expressed in the corolla, and its expression level increased significantly during the anthesis period. Dual luciferase assay revealed that PhbZIP3 could enhance the expression of PhPAL2 and PhBSMT. The findings from the yeast one-hybrid assay further corroborated the interaction between PhbZIP3 and the promoters of PhPAL2 and PhBSMT. Taken together, these results suggest that PhbZIP3 play a pivotal role in the regulatory mechanism of FVBP biosynthesis in petunia.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
盛宇大天才完成签到,获得积分10
2秒前
游戏人间完成签到 ,获得积分10
3秒前
4秒前
科研通AI5应助淡淡的忆彤采纳,获得10
4秒前
早日毕业完成签到,获得积分10
4秒前
Billie完成签到,获得积分10
5秒前
积极行天完成签到,获得积分10
5秒前
98完成签到,获得积分10
6秒前
nkmenghan完成签到,获得积分20
7秒前
韶邑完成签到,获得积分10
7秒前
penzer完成签到 ,获得积分10
8秒前
suwan完成签到,获得积分10
9秒前
张瀚文完成签到 ,获得积分10
12秒前
不吃香菜完成签到 ,获得积分10
14秒前
何日完成签到,获得积分10
16秒前
明天完成签到,获得积分10
16秒前
rrrick完成签到,获得积分10
16秒前
XF发布了新的文献求助10
17秒前
结实乐曲完成签到,获得积分10
17秒前
17秒前
18秒前
顺利紫山完成签到,获得积分10
19秒前
liaodongjun完成签到,获得积分10
20秒前
21秒前
ma完成签到,获得积分10
21秒前
GOW完成签到,获得积分10
22秒前
淡淡的忆彤完成签到,获得积分10
23秒前
23秒前
23秒前
songvv发布了新的文献求助10
24秒前
六沉完成签到 ,获得积分10
25秒前
爱笑的曼易完成签到,获得积分10
25秒前
爆炒菜头完成签到,获得积分10
25秒前
壮观的谷冬完成签到,获得积分10
25秒前
研友_VZG7GZ应助小王采纳,获得10
25秒前
imuzi完成签到,获得积分10
26秒前
tans0008完成签到,获得积分10
26秒前
霸气果汁完成签到,获得积分10
26秒前
程南完成签到,获得积分10
27秒前
JAMA兜里揣发布了新的文献求助10
27秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038303
求助须知:如何正确求助?哪些是违规求助? 3576013
关于积分的说明 11374210
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029