基因传递
材料科学
乙二醇
DNA缩合
共聚物
生物相容性
聚合物
偶氮苯
生物物理学
超分子化学
体内
赫拉
纳米技术
阳离子聚合
体外
遗传增强
化学
转染
高分子化学
基因
生物化学
有机化学
生物
分子
冶金
复合材料
生物技术
作者
Wumaier Yasen,Bei Li,Aliya Aini,Ziying Li,Yue Su,Linzhu Zhou,Dongbo Guo,Qiuhui Qian,Dong Chen,Xinyuan Zhu,Ruijiao Dong
标识
DOI:10.1021/acsami.3c06170
摘要
To achieve efficient gene delivery in vitro or in vivo, nonviral vectors should have excellent biostability across cellular and tissue barriers and also smart stimuli responsiveness toward controlled release of therapeutic genes into the cell nucleus. However, it remains a key challenge to effectively combine the biostability of covalent polymers with the stimuli responsiveness of noncovalent polymers into one nonviral vehicle. In this work, we report the construction of a kind of cationic supramolecular block copolymers (SBCs) through noncovalent polymerization of β-cyclodextrin/azobenzene-terminated pentaethylenehexamine (DMA-Azo-PEHA-β-CD) in aqueous media using β-CD-monosubstituted poly(ethylene glycol) (PEG-β-CD) as a supramolecular initiator. The resultant SBC exhibits superior biostability, biocompatibility, and light/pH dual-responsive characteristics, and it also demonstrates efficient plasmid DNA condensation capacity and the ability to rapidly release plasmid DNA into cells driven by visible light (450 nm). Eventually, this SBC-based delivery system demonstrates visible light-induced enhancement of gene delivery in both COS-7 and HeLa cells. We anticipate that this work provides a facile and robust strategy to enhance gene delivery in vitro or in vivovia visible light-guided manipulation of genes, further achieving safe, highly efficient, targeting gene therapy for cancer.
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