The temperature-dependent ON-state breakdown BVON loci of AlGaN/GaN high-electron-mobility transistors (HEMTs) with an AlGaN back barrier were investigated using the gate current extraction technique. The impact ionization of acceptor-like traps was revealed to be responsible for the ON-state breakdown in HEMTs as a 2D electron gas (2DEG) channel is marginally turned on. The characteristic electric field Ei of impact ionization was extracted, exhibiting a U-shaped temperature dependence from 40 to −30 ∘C, with minimum Ei occurring at −10 ∘C. The impurity scattering effect of acceptor-like traps in AlGaN/GaN heterostructures is suggested to be responsible for the negative temperature dependence of BVON and Ei below −10 ∘C.