Complex Duplications at 6p22.1, 6pll.2, 5q13, 5p15.1 and 5p13 Revealed by FluorescentIn SituHybridisation

AbstractA complex pattern of fluorescent in situ hybridisation (FISH) has been detected using PAC clones from the short arm of chromosome 6, proximal to the haemochromatosis gene at 6p22.1. Cross-hybridisation to 6p22.1, 6p11.2, 5q13, 5p15.1 and 5p13 was consistently detected with several PAC clones covering a genomic region greater than 200kb. These results indicate that large sections of genomic DNA are shared by these 5 disparate chromosomal segments, indicative of large scale duplication events. These results were in part accounted for by the identification of several expressed sequence tags (ESTs).Key Words: Haemochromatosischromosomes 6p22.16p11.25q13p15.15p13duplicationsmultigene family