Summary1. The mouse is a suitable animal for collection of thoracic duct lymph in the partially-restrained unanesthetized state. 2. Initial values for lymph flow and lymphocyte output did not vary significantly in the unanesthetized as compared to the anesthetized mouse. 3. A continuous decrease in lymph flow and lymphocyte output from the thoracic duct occurred over a 24-hour period in the unanesthetized mouse. 4. Values for thoracic duct lymph flow and lymphocyte output did not vary significantly, whether collected from the neck or abdomen.