Effects of humic acid on sludge performance, antibiotics resistance genes propagation and functional genes expression during Cu(II)-containing wastewater treatment via metagenomics analysis

The humic acid (HA) function on the sludge performance, antibiotics resistance genes (ARGs) propagation and functional genes expression during Cu(II)-containing wastewater treatment was comprehensively investigated via metagenomics analysis. Results showed that the pollutants removal was significantly inhibited after long-term exposure of 5 mg/L Cu(II), while the inhibitory effects were moderately alleviated after addition of 10 mg/L HA. The extracellular polymeric substances (EPS) production with Cu(II) acclimation was higher than the sludge with Cu(II) and HA acclimation. The microbial community was significantly affected by the HA addition, while the relative abundance of dominant ARGs had no distinct differences with or without HA addition under Cu(II) stress. The functional genes were largely implemented for microbial metabolism, while no significant differences were found with HA addition under Cu(II) stress. Thus, the HA function for ARGs propagation and functional genes expression needed to be further research under Cu(II) stress in wastewater treatment.