Sesaminol induces brown and beige adipocyte formation through suppression of myogenic program

The FASEB JournalVolume 34, Issue 5 p. 6854-6870 RESEARCH ARTICLEFree to Read Sesaminol induces brown and beige adipocyte formation through suppression of myogenic program Soumya Jaya Divakaran, Soumya Jaya Divakaran Cardiovascular Diseases and Diabetes Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, IndiaSearch for more papers by this authorSimran Srivastava, Simran Srivastava Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru, IndiaSearch for more papers by this authorAnusha Jahagirdar, Anusha Jahagirdar Cardiovascular Diseases and Diabetes Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, IndiaSearch for more papers by this authorRajprabu Rajendran, Rajprabu Rajendran Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru, IndiaSearch for more papers by this authorShinde Vijay Sukhdeo, Shinde Vijay Sukhdeo Department of Lipid Science, Lipidomics Center, CSIR-Central Food Technological Research Institute, Mysuru, IndiaSearch for more papers by this authorSona Rajakumari, Corresponding Author Sona Rajakumari [email protected] Cardiovascular Diseases and Diabetes Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru, India Correspondence Sona Rajakumari, Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru 560012, India. Email: [email protected]Search for more papers by this author Soumya Jaya Divakaran, Soumya Jaya Divakaran Cardiovascular Diseases and Diabetes Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, IndiaSearch for more papers by this authorSimran Srivastava, Simran Srivastava Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru, IndiaSearch for more papers by this authorAnusha Jahagirdar, Anusha Jahagirdar Cardiovascular Diseases and Diabetes Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, IndiaSearch for more papers by this authorRajprabu Rajendran, Rajprabu Rajendran Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru, IndiaSearch for more papers by this authorShinde Vijay Sukhdeo, Shinde Vijay Sukhdeo Department of Lipid Science, Lipidomics Center, CSIR-Central Food Technological Research Institute, Mysuru, IndiaSearch for more papers by this authorSona Rajakumari, Corresponding Author Sona Rajakumari [email protected] Cardiovascular Diseases and Diabetes Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru, India Correspondence Sona Rajakumari, Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru 560012, India. Email: [email protected]Search for more papers by this author First published: 10 April 2020 https://doi.org/10.1096/fj.201902124R Soumya Jaya Divakaran and Simran Srivastava are contributed equally to this work. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Adipocytes are key players in maintaining energy homeostasis and are classified into two different categories: white and brown adipocytes. While white adipocytes store energy as triacylglycerols in lipid droplets, brown adipocytes combust excess chemical energy and release in the form of heat through uncoupled respiration. This characteristic phenomenon of brown fat attracts researchers and pharmacological industries to view brown fat as one of the potential therapeutic targets for obesity and associated metabolic disease. In the current study, we investigated the effect of a small molecule, sesaminol (SML) on brown fat activity and found that SML induces the thermogenic program in primary white adipocytes as well as chow diet fed mice. In particular, SML treatment to mice elevated mitochondrial complex proteins and the rate of oxygen consumption in brown and white fat. Administration of SML to high fat diet (HFD) challenged mice decreased weight gain, adiposity and cholesterol levels along with an increase of brown fat gene program in brown and white fat. Mechanistically, SML repressed the myogenic gene program in C2C12 myoblasts and increased all mitochondrial marker genes as appeared in brown adipose cells. Together, our results demonstrate that SML stimulates brown adipose function and protects mice against diet-induced weight gain. CONFLICT OF INTEREST All authors listed here declare no conflict of interest. Supporting Information Filename Description fsb220466-sup-0001-Suppinfo.pdfPDF document, 4.1 MB Supplementary Material Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume34, Issue5May 2020Pages 6854-6870 RelatedInformation