类有机物
肝星状细胞
癌症研究
祖细胞
生物
诱导多能干细胞
医学
原发性硬化性胆管炎
细胞
细胞培养
电池类型
病理
细胞生物学
干细胞
胚胎干细胞
疾病
基因
生物化学
遗传学
作者
Keisaku Sato,Wenjun Zhang,Samira Safarikia,Abdulkadir Isidan,Angela M. Chen,Ping Li,Heather Francis,Lindsey Kennedy,Leonardo Baiocchi,Domenico Alvaro,Shannon Glaser,Burcin Ekser,Gianfranco Alpini
出处
期刊:Hepatology
[Wiley]
日期:2021-06-04
卷期号:74 (1): 491-502
被引量:42
摘要
Cholangiopathies, such as primary sclerosing cholangitis, biliary atresia, and cholangiocarcinoma, have limited experimental models. Not only cholangiocytes but also other hepatic cells including hepatic stellate cells and macrophages are involved in the pathophysiology of cholangiopathies, and these hepatic cells orchestrate the coordinated response against diseased conditions. Classic two‐dimensional monolayer cell cultures do not resemble intercellular cell‐to‐cell interaction and communication; however, three‐dimensional cell culture systems, such as organoids and spheroids, can mimic cellular interaction and architecture between hepatic cells. Previous studies have demonstrated the generation of hepatic or biliary organoids/spheroids using various cell sources including pluripotent stem cells, hepatic progenitor cells, primary cells from liver biopsies, and immortalized cell lines. Gene manipulation, such as transfection and transduction can be performed in organoids, and established organoids have functional characteristics which can be suitable for drug screening. This review summarizes current methodologies for organoid/spheroid formation and a potential for three‐dimensional hepatic cell cultures as in vitro models of cholangiopathies.
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