INSM1 Is Less Sensitive But More Specific Than Synaptophysin in Gynecologic High-grade Neuroendocrine Carcinomas

突触素 嗜铬粒蛋白A 病理 免疫组织化学 神经内分泌分化 神经内分泌肿瘤 组织微阵列 医学 内科学 生物 癌症 前列腺癌
作者
Qiong Zou,Lily Zhang,Zhiqiang Cheng,Xiaojing Guo,Dengfeng Cao
出处
期刊:The American Journal of Surgical Pathology [Lippincott Williams & Wilkins]
卷期号:45 (2): 147-159 被引量:24
标识
DOI:10.1097/pas.0000000000001641
摘要

Insulinoma-associated protein 1 (INSM1) has emerged as a promising diagnostic marker for high-grade neuroendocrine carcinomas (HGNECs); however, it is controversial whether INSM1 is more sensitive than conventional markers chromogranin, synaptophysin, and CD56. Here, we investigated immunohistochemical expression of INSM1 in 75 gynecologic HGNECs using full tissue sections (30 small-cell carcinomas [SmCCs], 34 large-cell neuroendocrine carcinomas [LCNECs], and 11 mixed SmCC and LCNEC), with specificity analysis in 422 gynecologic non-neuroendocrine tumors (410 in tissue microarrays and 12 full sections) and comparison with conventional neuroendocrine markers for their sensitivity and specificity. Positive INSM1 staining was seen in 69 (92%) HGNECs, whereas chromogranin, synaptophysin, and CD56 staining was seen in 61 (81%), 72 (96%), and 44 (69%) tumors, respectively (INSM1 vs. chromogranin, P =0.09; INSM1 vs. synaptophysin, P =0.4942; and INSM1 vs. CD56, P <0.001). The mean percentage of INSM1-positive tumor cells was 54% (median: 60%, range: 0% to 100%), similar to chromogranin (58%, P =0.2903) and higher than CD56 (30%, P =0.00001) but significantly lower than synaptophysin (89%, P <0.00001). INSM1 showed no staining difference among SmCCs, LCNECs, and mixed SmCC-LCNECs. Among the 422 non-neuroendocrine tumors, positive staining was seen in 5% tumors for INSM1, 18% for chromogranin, 19% for synaptophysin, and 25% for CD56. Our study indicates that INSM1 is a highly specific marker (95% specificity) for gynecologic HGNECs with high sensitivity (92%), but it is less sensitive than synaptophysin (96% sensitivity). INSM1 is more specific than chromogranin, synaptophysin, and CD56 for gynecologic HGNECs. Our literature review reveals that INSM1 has consistently (the same antibody clone A8 used for all reported studies) shown higher or similar sensitivity to chromogranin (for all 3 chromogranin antibody clones LK2H10, DAK-A3, DAKO polyclonal); however, whether INSM1 is more or less sensitive than synaptophysin or CD56 for HGNECs is highly dependent on the antibody clones used for synaptophysin (clones MRQ-40 and SNP88 showing higher sensitivity than clones 27G12 and DAK-SYNAP) or CD56 (clones CD564, MRQ-42, and MRQ-54 showing higher sensitivity than clones 123C3D5, 1B6, and Leu243).
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
火星上小土豆完成签到 ,获得积分10
7秒前
杰尼龟的鱼完成签到 ,获得积分10
11秒前
安然完成签到 ,获得积分10
12秒前
张希伦完成签到 ,获得积分10
12秒前
任性翠安完成签到 ,获得积分10
16秒前
dong完成签到 ,获得积分10
18秒前
神说完成签到,获得积分0
20秒前
量子星尘发布了新的文献求助10
26秒前
Aimee完成签到 ,获得积分10
26秒前
徐彬荣完成签到,获得积分10
27秒前
研友_8yN60L完成签到,获得积分10
28秒前
搜集达人应助科研通管家采纳,获得10
28秒前
光亮的自行车完成签到 ,获得积分10
28秒前
李东东完成签到 ,获得积分10
43秒前
王多肉完成签到,获得积分10
47秒前
Iiiilr完成签到 ,获得积分10
48秒前
杨幂完成签到,获得积分10
49秒前
56秒前
hellokitty完成签到,获得积分10
59秒前
59秒前
小四发布了新的文献求助10
1分钟前
1分钟前
西瓜完成签到 ,获得积分10
1分钟前
包容的忆灵完成签到 ,获得积分10
1分钟前
高兴尔冬发布了新的文献求助10
1分钟前
xiang完成签到 ,获得积分0
1分钟前
小四完成签到,获得积分10
1分钟前
FashionBoy应助slayers采纳,获得30
1分钟前
量子星尘发布了新的文献求助10
1分钟前
黑眼圈完成签到 ,获得积分10
1分钟前
jia完成签到 ,获得积分10
1分钟前
如履平川完成签到 ,获得积分10
1分钟前
科目三应助忧伤的步美采纳,获得10
1分钟前
大椒完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
wisdom完成签到,获得积分10
1分钟前
slayers发布了新的文献求助30
1分钟前
1分钟前
e746700020完成签到,获得积分10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038039
求助须知:如何正确求助?哪些是违规求助? 3575756
关于积分的说明 11373782
捐赠科研通 3305574
什么是DOI,文献DOI怎么找? 1819239
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022