Comparative transcriptome analysis reveals candidate genes involved in anthocyanin biosynthesis in sweetpotato (Ipomoea batatas L.)

花青素 甘薯 肉体 生物 转录组 基因 旋花科 候选基因 栽培 植物 园艺 基因表达 遗传学
作者
Qiang Li,Meng Kou,Chen Li,Yungang Zhang
出处
期刊:Plant Physiology and Biochemistry [Elsevier BV]
卷期号:158: 508-517 被引量:22
标识
DOI:10.1016/j.plaphy.2020.11.035
摘要

Sweetpotato [Ipomoea batatas (L.) Lam] is an economically important crop for fresh and processed consumption and is widely cultivated worldwide, especially in China. Various sweetpotato cultivars with different storage root colors are presently available. The purple-fleshed sweetpotato obtains its color from anthocyanin accumulation in the storage roots, which is beneficial for both plant and human health. To date, the molecular mechanism of this anthocyanin accumulation has not been studied in detail. In our study, three cDNA libraries generated from ‘Xuzi8’ with dark-purple flesh, ‘Xuzi6’ with light-purple flesh, and ‘Xu28’ with white flesh were sequenced utilizing an Illumina HiSeq™ 2500 platform. Corresponding totals of 28,093,466, 29,239,729 and 27,217,440 raw reads were obtained from the three libraries and assembled into 137,625 unigenes with an average length of 481 bp. Moreover, 79,203 unigenes (57.55%) were found to be annotated in several public databases, and 1285 unigenes were differentially expressed among the Xu28 vs Xuzi8, Xu28 vs Xuzi6, and Xuzi6 vs Xuzi8 libraries. After functional category enrichment analysis of differential expression genes (DEGs), 25 genes were selected as the candidate genes related to anthocyanin accumulation. Furthermore, the expression patterns of some selected DEGs were verified by quantitative real-time PCR (qRT-PCR), and the correlation between expression levels of relevant genes involved in anthocyanin biosynthesis and anthocyanin content was determined. Taken together, the results compose a transcriptomic analysis to investigate the differences in purple flesh formation in the storage roots among different sweetpotato varieties, with the notable outcome that several key genes can now be closely linked to anthocyanin biosynthesis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI5应助科研通管家采纳,获得10
刚刚
科目三应助科研通管家采纳,获得10
1秒前
爆米花应助科研通管家采纳,获得10
1秒前
稳重淇完成签到 ,获得积分10
1秒前
1秒前
2秒前
3秒前
daaarrr完成签到,获得积分10
6秒前
非一发布了新的文献求助10
9秒前
9秒前
10秒前
11秒前
乐乐应助事在人为采纳,获得10
12秒前
13秒前
jisujun完成签到,获得积分20
13秒前
41应助momo采纳,获得10
14秒前
DijiaXu应助jszhoucl采纳,获得10
16秒前
热爱科研的小白鼠完成签到,获得积分10
16秒前
16秒前
爱穿毛袜完成签到,获得积分10
17秒前
大模型应助LJJ采纳,获得10
17秒前
spirit完成签到 ,获得积分10
18秒前
思源应助hhh采纳,获得10
18秒前
19秒前
正直的魔镜完成签到 ,获得积分10
20秒前
22秒前
KM比比发布了新的文献求助10
23秒前
如此完成签到,获得积分10
23秒前
qq完成签到 ,获得积分10
24秒前
qqq发布了新的文献求助10
25秒前
火星上鑫鹏完成签到,获得积分10
25秒前
事在人为发布了新的文献求助10
25秒前
沉默的婴发布了新的文献求助20
26秒前
杨涵完成签到 ,获得积分10
27秒前
ponysmile完成签到,获得积分20
28秒前
葡萄完成签到,获得积分10
28秒前
28秒前
共享精神应助卖萌的秋田采纳,获得10
29秒前
30秒前
阿钉发布了新的文献求助10
32秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989334
求助须知:如何正确求助?哪些是违规求助? 3531428
关于积分的说明 11253936
捐赠科研通 3270119
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809173